LAM-B
Catalog Fields
Clone ID/Product Name: LAM-B
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_528340
Clonality: Monoclonal
Immunogen: Mouse yolk sac carcinoma cells derived from NF-1 teratocarcinoma.
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: P3-X63-Ag-8.653
Epitope Mapped: No
Antigen Name: laminin, murine parietal yolk sac
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/3/1992
Antigen Molecular Weight: Apparent: 200 kDa (beta and gamma) and 400 kDa (alpha)
Depositor: Damjanov, I.
Antigen Sequence:
Depositor Institution: Kansas University Medical Center
Antigen Species: Mouse
Depositor Notes: Mouse laminin, yolk sac specific. This antibody reacts only with parietal yolk sac laminin, 6-18 day yolk sac of mouse; does not react with adult basement membrane or rat yolk sac.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: Immunoblotting: LAM-B doesn't work well on denatured laminin.
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LAM-B was deposited to the DSHB by Damjanov, I. (DSHB Hybridoma Product LAM-B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
3 References
Initial Publication
Distinct antigenic characteristics of murine parietal yolk sac laminin.
Damjanov I
Developmental biology 121.2 (1987 Jun): 397-407.