5E1

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: 5E1
Available to For-Profits: Yes
Alternate Antibody Name: m5E1, alpha-SHH
Gene Symbol: Shh
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528466 
Uniprot ID: Q63673 
RRID:  
Entrez Gene ID: 29499 
Clonality: Monoclonal
Immunogen: synthetic peptide
Clone:
Immunogen Sequence: N-terminal SSH fragment
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: sonic hedgehog
Epitope Location or Sequence: aa. 157-178
Alternate Antigen Name:
Deposit Date: 12/3/1996
Antigen Molecular Weight: 20 kDa SHH-N protein
Depositor: Jessell, T.M. / Brenner-Morton, S.
Antigen Sequence: sonic hedgehog N-terminal SSH fragment 1-198 from rat
Depositor Institution: Columbia University / HHMI
Antigen Species: Rat
Depositor Notes: Ligand for the Patched receptor
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Lizard, Mouse, Quail, Rabbit, Rat
Additional Information: Also recognizes IHH (Indian Hedghog) PMID: 8662546 . The functionally blocked receptor is ptc. Does not bind to ShhC25S protein.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5E1 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 5E1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

43 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
    • Initial Publication

    Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity.
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    IF References

    Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification.
    George-Weinstein M
    Developmental biology 359.1 (2011 Nov 1): 12-25.

    Sonic hedgehog is associated with H+-K+-ATPase-containing membranes in gastric parietal cells and secreted with histamine stimulation.
    Malinowska DH
    American journal of physiology. Gastrointestinal and liver physiology 295.1 (2008 Jul): G99-G111.

    The upregulated expression of sonic hedgehog in motor neurons after rat facial nerve axotomy.
    Kohsaka S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.36 (2004 Sep 8): 7923-30.

    Phosphoinositide 3-kinase and Akt are essential for Sonic Hedgehog signaling.
    Emerson CP Jr
    Proceedings of the National Academy of Sciences of the United States of America 103.12 (2006 Mar 21): 4505-10.

    Temporal progression of hypothalamic patterning by a dual action of BMP.
    Placzek M
    Development (Cambridge, England) 135.20 (2008 Oct): 3325-31.

    Temporal progression of hypothalamic patterning by a dual action of BMP.
    Placzek M
    Development (Cambridge, England) 135.20 (2008 Oct): 3325-31.

    Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development.
    Lin J
    Journal of molecular neuroscience : MN 64.2 (2018 Feb): 287-299.

    Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development.
    Lin J
    Journal of molecular neuroscience : MN 64.2 (2018 Feb): 287-299.

    Scaling Pattern to Variations in Size during Development of the Vertebrate Neural Tube.
    Tabin CJ
    Developmental cell 37.2 (2016 Apr 18): 127-35.

    Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center.
    Roelink H
    BMC molecular and cell biology 22.1 (2021 Apr 16): 22.

    WB References

    Molecular pathways regulating pro-migratory effects of Hedgehog signaling.
    Izraeli S
    The Journal of biological chemistry 281.45 (2006 Nov 10): 33860-70.

    Indian hedgehog gene transfer augments hematopoietic support of human stromal cells including NOD/SCID-beta2m-/- repopulating cells.
    Hamada H
    Blood 104.4 (2004 Aug 15): 1002-9.

    IHC References

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
    Oláh I
    Development (Cambridge, England) 137.18 (2010 Sep): 3019-23.

    Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.
    Hymowitz SG
    The Journal of biological chemistry 285.34 (2010 Aug 20): 26570-80.

    Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.
    Stoeckli ET
    Nucleic acids research 39.20 (2011 Nov 1): e133.

    Epithelial hedgehog signals pattern the intestinal crypt-villus axis.
    Gumucio DL
    Development (Cambridge, England) 132.2 (2005 Jan): 279-89.

    Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
    Kaprielian Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.35 (2008 Aug 27): 8698-708.

    Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh.
    Nicolis SK
    Nature neuroscience 12.10 (2009 Oct): 1248-56.

    ARL13B regulates Sonic hedgehog signaling from outside primary cilia.
    Caspary T
    eLife 9. (2020 Mar 4): .

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    IP References

    Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development.
    Lin X
    PLoS genetics 13.8 (2017 Aug): e1006992.

    FB References

    Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity.
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Hedgehog signaling regulates differentiation from double-negative to double-positive thymocyte.
    Crompton T
    Immunity 13.2 (2000 Aug): 187-97.

    Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis.
    Yang XJ
    Developmental biology 233.2 (2001 May 15): 271-90.

    Inhibition of prostate cancer proliferation by interference with SONIC HEDGEHOG-GLI1 signaling.
    Ruiz i Altaba A
    Proceedings of the National Academy of Sciences of the United States of America 101.34 (2004 Aug 24): 12561-6.

    Hedgehog signaling regulates MyoD expression and activity.
    Skerjanc IS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4389-404.

    Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.
    Hymowitz SG
    The Journal of biological chemistry 285.34 (2010 Aug 20): 26570-80.

    A role for Sonic hedgehog in axon-to-astrocyte signalling in the rodent optic nerve.
    Raff MC
    Development (Cambridge, England) 126.13 (1999 Jul): 2901-9.

    Purkinje-cell-derived Sonic hedgehog regulates granule neuron precursor cell proliferation in the developing mouse cerebellum.
    Wallace VA
    Current biology : CB 9.8 (1999 Apr 22): 445-8.

    Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours.
    Beachy PA
    Nature 425.6960 (2003 Oct 23): 846-51.

    Chemoattractive activity of sonic hedgehog in the adult subventricular zone modulates the number of neural precursors reaching the olfactory bulb.
    Traiffort E
    Stem cells (Dayton, Ohio) 26.9 (2008 Sep): 2311-20.

    A paracrine requirement for hedgehog signalling in cancer.
    de Sauvage FJ
    Nature 455.7211 (2008 Sep 18): 406-10.

    A freely diffusible form of Sonic hedgehog mediates long-range signalling.
    Robbins DJ
    Nature 411.6838 (2001 Jun 7): 716-20.

    Treatment of patients with HIV thrombocytopenia and hemolytic uremic syndrome with protein A (Prosorba column) immunoadsorption.
    Arlin Z
    Seminars in hematology 26.2 Suppl 1 (1989 Apr): 15-8.

    Sonic hedgehog shedding results in functional activation of the solubilized protein.
    Grobe K
    Developmental cell 20.6 (2011 Jun 14): 764-74.

    Dual roles of the Cardin-Weintraub motif in multimeric Sonic hedgehog.
    Grobe K
    The Journal of biological chemistry 286.26 (2011 Jul 1): 23608-19.

    Interactions between Shh, Sostdc1 and Wnt signaling and a new feedback loop for spatial patterning of the teeth.
    Jung HS
    Development (Cambridge, England) 138.9 (2011 May): 1807-16.

    Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear.
    Wu DK
    Development (Cambridge, England) 132.9 (2005 May): 2115-24.

    Molecular pathways regulating pro-migratory effects of Hedgehog signaling.
    Izraeli S
    The Journal of biological chemistry 281.45 (2006 Nov 10): 33860-70.

    Sonic hedgehog promotes CD4+ T lymphocyte proliferation and modulates the expression of a subset of CD28-targeted genes.
    Tam PK
    International immunology 18.12 (2006 Dec): 1627-36.

    Chondrocytic Atf4 regulates osteoblast differentiation and function via Ihh.
    Yang X
    Development (Cambridge, England) 139.3 (2012 Feb): 601-11.

    Drug resistance is dramatically restored by hedgehog inhibitors in CD34+ leukemic cells.
    Kato J
    Cancer science 100.5 (2009 May): 948-55.

    Hedgehog signaling maintains a tumor stem cell compartment in multiple myeloma.
    Matsui W
    Proceedings of the National Academy of Sciences of the United States of America 104.10 (2007 Mar 6): 4048-53.

    Shh-dependent formation of the ZLI is opposed by signals from the dorsal diencephalon.
    Zeltser LM
    Development (Cambridge, England) 132.9 (2005 May): 2023-33.

    Indian hedgehog gene transfer augments hematopoietic support of human stromal cells including NOD/SCID-beta2m-/- repopulating cells.
    Hamada H
    Blood 104.4 (2004 Aug 15): 1002-9.

    Shox2-deficient mice exhibit a rare type of incomplete clefting of the secondary palate.
    Chen Y
    Development (Cambridge, England) 132.19 (2005 Oct): 4397-406.

    Tissue-derived hedgehog proteins modulate Th differentiation and disease.
    Crompton T
    Journal of immunology (Baltimore, Md. : 1950) 190.6 (2013 Mar 15): 2641-9.

    Epitope Map References

    Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.
    Hymowitz SG
    The Journal of biological chemistry 285.34 (2010 Aug 20): 26570-80.

    Dual roles of the Cardin-Weintraub motif in multimeric Sonic hedgehog.
    Grobe K
    The Journal of biological chemistry 286.26 (2011 Jul 1): 23608-19.

    Sonic hedgehog shedding results in functional activation of the solubilized protein.
    Grobe K
    Developmental cell 20.6 (2011 Jun 14): 764-74.

    All References

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
    Oláh I
    Development (Cambridge, England) 137.18 (2010 Sep): 3019-23.

    Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.
    Hymowitz SG
    The Journal of biological chemistry 285.34 (2010 Aug 20): 26570-80.

    Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.
    Stoeckli ET
    Nucleic acids research 39.20 (2011 Nov 1): e133.

    Epithelial hedgehog signals pattern the intestinal crypt-villus axis.
    Gumucio DL
    Development (Cambridge, England) 132.2 (2005 Jan): 279-89.

    Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
    Kaprielian Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.35 (2008 Aug 27): 8698-708.

    Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh.
    Nicolis SK
    Nature neuroscience 12.10 (2009 Oct): 1248-56.

    ARL13B regulates Sonic hedgehog signaling from outside primary cilia.
    Caspary T
    eLife 9. (2020 Mar 4): .

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification.
    George-Weinstein M
    Developmental biology 359.1 (2011 Nov 1): 12-25.

    Sonic hedgehog is associated with H+-K+-ATPase-containing membranes in gastric parietal cells and secreted with histamine stimulation.
    Malinowska DH
    American journal of physiology. Gastrointestinal and liver physiology 295.1 (2008 Jul): G99-G111.

    The upregulated expression of sonic hedgehog in motor neurons after rat facial nerve axotomy.
    Kohsaka S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.36 (2004 Sep 8): 7923-30.

    Phosphoinositide 3-kinase and Akt are essential for Sonic Hedgehog signaling.
    Emerson CP Jr
    Proceedings of the National Academy of Sciences of the United States of America 103.12 (2006 Mar 21): 4505-10.

    Temporal progression of hypothalamic patterning by a dual action of BMP.
    Placzek M
    Development (Cambridge, England) 135.20 (2008 Oct): 3325-31.

    Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development.
    Lin J
    Journal of molecular neuroscience : MN 64.2 (2018 Feb): 287-299.

    Scaling Pattern to Variations in Size during Development of the Vertebrate Neural Tube.
    Tabin CJ
    Developmental cell 37.2 (2016 Apr 18): 127-35.

    Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center.
    Roelink H
    BMC molecular and cell biology 22.1 (2021 Apr 16): 22.

    Molecular pathways regulating pro-migratory effects of Hedgehog signaling.
    Izraeli S
    The Journal of biological chemistry 281.45 (2006 Nov 10): 33860-70.

    Indian hedgehog gene transfer augments hematopoietic support of human stromal cells including NOD/SCID-beta2m-/- repopulating cells.
    Hamada H
    Blood 104.4 (2004 Aug 15): 1002-9.

    Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development.
    Lin X
    PLoS genetics 13.8 (2017 Aug): e1006992.

    Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity.
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Hedgehog signaling regulates differentiation from double-negative to double-positive thymocyte.
    Crompton T
    Immunity 13.2 (2000 Aug): 187-97.

    Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis.
    Yang XJ
    Developmental biology 233.2 (2001 May 15): 271-90.

    Inhibition of prostate cancer proliferation by interference with SONIC HEDGEHOG-GLI1 signaling.
    Ruiz i Altaba A
    Proceedings of the National Academy of Sciences of the United States of America 101.34 (2004 Aug 24): 12561-6.

    Hedgehog signaling regulates MyoD expression and activity.
    Skerjanc IS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4389-404.

    A role for Sonic hedgehog in axon-to-astrocyte signalling in the rodent optic nerve.
    Raff MC
    Development (Cambridge, England) 126.13 (1999 Jul): 2901-9.

    Purkinje-cell-derived Sonic hedgehog regulates granule neuron precursor cell proliferation in the developing mouse cerebellum.
    Wallace VA
    Current biology : CB 9.8 (1999 Apr 22): 445-8.

    Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours.
    Beachy PA
    Nature 425.6960 (2003 Oct 23): 846-51.

    Chemoattractive activity of sonic hedgehog in the adult subventricular zone modulates the number of neural precursors reaching the olfactory bulb.
    Traiffort E
    Stem cells (Dayton, Ohio) 26.9 (2008 Sep): 2311-20.

    A paracrine requirement for hedgehog signalling in cancer.
    de Sauvage FJ
    Nature 455.7211 (2008 Sep 18): 406-10.

    A freely diffusible form of Sonic hedgehog mediates long-range signalling.
    Robbins DJ
    Nature 411.6838 (2001 Jun 7): 716-20.

    Treatment of patients with HIV thrombocytopenia and hemolytic uremic syndrome with protein A (Prosorba column) immunoadsorption.
    Arlin Z
    Seminars in hematology 26.2 Suppl 1 (1989 Apr): 15-8.

    Sonic hedgehog shedding results in functional activation of the solubilized protein.
    Grobe K
    Developmental cell 20.6 (2011 Jun 14): 764-74.

    Dual roles of the Cardin-Weintraub motif in multimeric Sonic hedgehog.
    Grobe K
    The Journal of biological chemistry 286.26 (2011 Jul 1): 23608-19.

    Interactions between Shh, Sostdc1 and Wnt signaling and a new feedback loop for spatial patterning of the teeth.
    Jung HS
    Development (Cambridge, England) 138.9 (2011 May): 1807-16.

    Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear.
    Wu DK
    Development (Cambridge, England) 132.9 (2005 May): 2115-24.

    Sonic hedgehog promotes CD4+ T lymphocyte proliferation and modulates the expression of a subset of CD28-targeted genes.
    Tam PK
    International immunology 18.12 (2006 Dec): 1627-36.

    Chondrocytic Atf4 regulates osteoblast differentiation and function via Ihh.
    Yang X
    Development (Cambridge, England) 139.3 (2012 Feb): 601-11.

    Drug resistance is dramatically restored by hedgehog inhibitors in CD34+ leukemic cells.
    Kato J
    Cancer science 100.5 (2009 May): 948-55.

    Hedgehog signaling maintains a tumor stem cell compartment in multiple myeloma.
    Matsui W
    Proceedings of the National Academy of Sciences of the United States of America 104.10 (2007 Mar 6): 4048-53.

    Shh-dependent formation of the ZLI is opposed by signals from the dorsal diencephalon.
    Zeltser LM
    Development (Cambridge, England) 132.9 (2005 May): 2023-33.

    Shox2-deficient mice exhibit a rare type of incomplete clefting of the secondary palate.
    Chen Y
    Development (Cambridge, England) 132.19 (2005 Oct): 4397-406.

    Tissue-derived hedgehog proteins modulate Th differentiation and disease.
    Crompton T
    Journal of immunology (Baltimore, Md. : 1950) 190.6 (2013 Mar 15): 2641-9.

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