M3F7
Catalog Fields
Product Name/ID: M3F7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: COL4A1/COL4A2
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 1282
Clonality: Monoclonal
Immunogen: Human placenta
Clone:
Immunogen Sequence: alpha1 (IV)-alpha2(IV) triple helix
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Collagen, type IV, alpha 1/ collagen, type IV alpha 2
Epitope Location or Sequence: Triple helical domain about 900 Å from the N-terminus
Alternate Antigen Name:
Deposit Date: 8/25/1988
Antigen Molecular Weight: 100 kDa/ 50 kDa
Depositor: Furthmayr, H.
Antigen Sequence:
Depositor Institution: Yale University
Antigen Species: Human
Depositor Notes: M3F7 recognizes type IV collagen in basement membranes in kidney, lung, placenta, cornea and skin. This antibody does not recognize denatured type IV collagen.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rat
Additional Information: RRID:AB_528167
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M3F7 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product M3F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
29 References
Initial Publication
Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
Furthmayr H
Laboratory investigation; a journal of technical methods and pathology 48.5 (1983 May): 639-49.