rat-401

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SKU: Rat-401-s

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: rat-401
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Nes
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2235915 
Uniprot ID: P21263 
RRID:  
Entrez Gene ID: 25491 
Clonality: Monoclonal
Immunogen: Spinal cord homogenate
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Nestin (intermediate filament protein)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/15/1993
Antigen Molecular Weight: 208.7 kDa
Depositor: Hockfield, S.
Antigen Sequence:
Depositor Institution: Yale University School of Medicine
Antigen Species: Rat
Depositor Notes: Nestin is expressed by progenitor glial cells in the CNS, Schwann cells in the PNS and early myoblast/myotubes.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rat-401 was deposited to the DSHB by Hockfield, S. (DSHB Hybridoma Product rat-401)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

36 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FACS References
  • All References
    • Initial Publication

    Identification of major cell classes in the developing mammalian nervous system.
    McKay RD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 5.12 (1985 Dec): 3310-28.

    IF References

    Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.
    Wolfe JH
    Stem cells translational medicine 2.6 (2013 Jun): 444-54.

    Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers.
    Leonhardt H
    Nucleic acids research 40.12 (2012 Jul): 5368-77.

    All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neurons.
    Martinez-Ceballos E
    Differentiation; research in biological diversity 83.5 (2012 Jun): 233-41.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    Efficient differentiation of embryonic stem cells into mesodermal precursors by BMP, retinoic acid and Notch signalling.
    Watt FM
    PloS one 7.4 (2012): e36405.

    Uni-directional differentiation of mouse embryonic stem cells into neurons by the neural stem sphere method.
    Inoue N
    Neuroscience research 69.4 (2011 Apr): 314-21.

    The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature.
    Okano H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 58.8 (2010 Aug): 721-30.

    The nuclear periphery of embryonic stem cells is a transcriptionally permissive and repressive compartment.
    Shopland LS
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3729-37.

    Identification of neural crest-derived stem cell-like cells from the corneal limbus of juvenile mice.
    Morsczeck C
    Experimental eye research 89.2 (2009 Aug): 209-17.

    Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Brüstle O
    Stem cells (Dayton, Ohio) 25.4 (2007 Apr): 939-49.

    Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
    Doetsch F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.11 (2015 Mar 18): 4528-39.

    Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status.
    Mather ML
    Scientific reports 8.1 (2018 Jan 15): 697.

    Aligned contiguous microfiber platform enhances neural differentiation of embryonic stem cells.
    Hu Z
    Scientific reports 8.1 (2018 Apr 17): 6087.

    Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive Pluripotency.
    Huber W
    Cell systems 7.5 (2018 Nov 28): 482-495.e10.

    Characterization of primary adult mouse cardiac fibroblast cultures.
    Pasumarthi KBS
    Canadian journal of physiology and pharmacology 98.12 (2020 Dec): 861-869.

    Postnatal Changes of Neural Stem Cells in the Mammalian Auditory Cortex.
    Deng M
    International journal of molecular sciences 22.4 (2021 Feb 4): .

    Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.
    Shibata M
    PloS one 16.4 (2021): e0249954.

    Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer's Disease.
    Soto C
    Cells 10.7 (2021 Jul 16): .

    WB References

    Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis.
    Lopez-Toledano MA
    International journal of Alzheimer's disease 2011. (2011): 929042.

    Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia.
    Goligorsky MS
    Kidney international 71.8 (2007 Apr): 744-54.

    EphA receptors direct the differentiation of mammalian neural precursor cells through a mitogen-activated protein kinase-dependent pathway.
    Tohyama M
    The Journal of biological chemistry 279.31 (2004 Jul 30): 32643-50.

    IHC References

    Enteric nervous system abnormalities are present in human necrotizing enterocolitis: potential neurotransplantation therapy.
    Besner GE
    Stem cell research & therapy 4.6 (2013): 157.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    N-cadherin regulates the proliferation and differentiation of ventral midbrain dopaminergic progenitors.
    Miyamoto Y
    Developmental neurobiology 73.7 (2013 Jul): 518-29.

    Dynamics of 5-hydroxymethylcytosine and chromatin marks in Mammalian neurogenesis.
    Lu Q
    Cell reports 3.2 (2013 Feb 21): 291-300.

    Dicer is required for proliferation, viability, migration and differentiation in corticoneurogenesis.
    Davidson BL
    Neuroscience 223. (2012 Oct 25): 285-95.

    Functional dicer is necessary for appropriate specification of radial glia during early development of mouse telencephalon.
    Price DJ
    PloS one 6.8 (2011): e23013.

    Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon-mediated gene transfer system.
    Miura M
    Genes to cells : devoted to molecular & cellular mechanisms 15.5 (2010 May): 501-12.

    Delayed dopaminergic neuron differentiation in Lrp6 mutant mice.
    Arenas E
    Developmental dynamics : an official publication of the American Association of Anatomists 239.1 (2010 Jan): 211-21.

    Efficient gene targeting by homologous recombination in rat embryonic stem cells.
    Burdon T
    PloS one 5.12 (2010 Dec 3): e14225.

    Promotion of proliferation in the developing cerebral cortex by EphA4 forward signaling.
    Donoghue MJ
    Development (Cambridge, England) 136.14 (2009 Jul): 2467-76.

    Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice.
    Parnavelas JG
    The Journal of comparative neurology 524.3 (2016 Feb 15): 518-34.

    Characterization of primary adult mouse cardiac fibroblast cultures.
    Pasumarthi KBS
    Canadian journal of physiology and pharmacology 98.12 (2020 Dec): 861-869.

    Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.
    Pollard SM
    Cell 184.9 (2021 Apr 29): 2454-2470.e26.

    Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer's Disease.
    Soto C
    Cells 10.7 (2021 Jul 16): .

    Ddx20, an Olig2 binding factor, governs the survival of neural and oligodendrocyte progenitor cells via proper Mdm2 splicing and p53 suppression.
    Takebayashi H
    Cell death and differentiation 29.5 (2022 May): 1028-1041.

    ELISA References

    Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis.
    Lopez-Toledano MA
    International journal of Alzheimer's disease 2011. (2011): 929042.

    FACS References

    Efficient differentiation of embryonic stem cells into mesodermal precursors by BMP, retinoic acid and Notch signalling.
    Watt FM
    PloS one 7.4 (2012): e36405.

    Notch exhibits ligand bias and maneuvers stage-specific steering of neural differentiation in embryonic stem cells.
    Lenka N
    Molecular and cellular biology 30.8 (2010 Apr): 1946-57.

    Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive Pluripotency.
    Huber W
    Cell systems 7.5 (2018 Nov 28): 482-495.e10.

    All References

    Enteric nervous system abnormalities are present in human necrotizing enterocolitis: potential neurotransplantation therapy.
    Besner GE
    Stem cell research & therapy 4.6 (2013): 157.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    N-cadherin regulates the proliferation and differentiation of ventral midbrain dopaminergic progenitors.
    Miyamoto Y
    Developmental neurobiology 73.7 (2013 Jul): 518-29.

    Dynamics of 5-hydroxymethylcytosine and chromatin marks in Mammalian neurogenesis.
    Lu Q
    Cell reports 3.2 (2013 Feb 21): 291-300.

    Dicer is required for proliferation, viability, migration and differentiation in corticoneurogenesis.
    Davidson BL
    Neuroscience 223. (2012 Oct 25): 285-95.

    Functional dicer is necessary for appropriate specification of radial glia during early development of mouse telencephalon.
    Price DJ
    PloS one 6.8 (2011): e23013.

    Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon-mediated gene transfer system.
    Miura M
    Genes to cells : devoted to molecular & cellular mechanisms 15.5 (2010 May): 501-12.

    Delayed dopaminergic neuron differentiation in Lrp6 mutant mice.
    Arenas E
    Developmental dynamics : an official publication of the American Association of Anatomists 239.1 (2010 Jan): 211-21.

    Efficient gene targeting by homologous recombination in rat embryonic stem cells.
    Burdon T
    PloS one 5.12 (2010 Dec 3): e14225.

    Promotion of proliferation in the developing cerebral cortex by EphA4 forward signaling.
    Donoghue MJ
    Development (Cambridge, England) 136.14 (2009 Jul): 2467-76.

    Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice.
    Parnavelas JG
    The Journal of comparative neurology 524.3 (2016 Feb 15): 518-34.

    Characterization of primary adult mouse cardiac fibroblast cultures.
    Pasumarthi KBS
    Canadian journal of physiology and pharmacology 98.12 (2020 Dec): 861-869.

    Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.
    Pollard SM
    Cell 184.9 (2021 Apr 29): 2454-2470.e26.

    Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer's Disease.
    Soto C
    Cells 10.7 (2021 Jul 16): .

    Ddx20, an Olig2 binding factor, governs the survival of neural and oligodendrocyte progenitor cells via proper Mdm2 splicing and p53 suppression.
    Takebayashi H
    Cell death and differentiation 29.5 (2022 May): 1028-1041.

    Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.
    Wolfe JH
    Stem cells translational medicine 2.6 (2013 Jun): 444-54.

    Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers.
    Leonhardt H
    Nucleic acids research 40.12 (2012 Jul): 5368-77.

    All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neurons.
    Martinez-Ceballos E
    Differentiation; research in biological diversity 83.5 (2012 Jun): 233-41.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    Efficient differentiation of embryonic stem cells into mesodermal precursors by BMP, retinoic acid and Notch signalling.
    Watt FM
    PloS one 7.4 (2012): e36405.

    Uni-directional differentiation of mouse embryonic stem cells into neurons by the neural stem sphere method.
    Inoue N
    Neuroscience research 69.4 (2011 Apr): 314-21.

    The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature.
    Okano H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 58.8 (2010 Aug): 721-30.

    The nuclear periphery of embryonic stem cells is a transcriptionally permissive and repressive compartment.
    Shopland LS
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3729-37.

    Identification of neural crest-derived stem cell-like cells from the corneal limbus of juvenile mice.
    Morsczeck C
    Experimental eye research 89.2 (2009 Aug): 209-17.

    Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Brüstle O
    Stem cells (Dayton, Ohio) 25.4 (2007 Apr): 939-49.

    Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
    Doetsch F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.11 (2015 Mar 18): 4528-39.

    Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status.
    Mather ML
    Scientific reports 8.1 (2018 Jan 15): 697.

    Aligned contiguous microfiber platform enhances neural differentiation of embryonic stem cells.
    Hu Z
    Scientific reports 8.1 (2018 Apr 17): 6087.

    Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive Pluripotency.
    Huber W
    Cell systems 7.5 (2018 Nov 28): 482-495.e10.

    Postnatal Changes of Neural Stem Cells in the Mammalian Auditory Cortex.
    Deng M
    International journal of molecular sciences 22.4 (2021 Feb 4): .

    Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.
    Shibata M
    PloS one 16.4 (2021): e0249954.

    Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis.
    Lopez-Toledano MA
    International journal of Alzheimer's disease 2011. (2011): 929042.

    Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia.
    Goligorsky MS
    Kidney international 71.8 (2007 Apr): 744-54.

    EphA receptors direct the differentiation of mammalian neural precursor cells through a mitogen-activated protein kinase-dependent pathway.
    Tohyama M
    The Journal of biological chemistry 279.31 (2004 Jul 30): 32643-50.

    Notch exhibits ligand bias and maneuvers stage-specific steering of neural differentiation in embryonic stem cells.
    Lenka N
    Molecular and cellular biology 30.8 (2010 Apr): 1946-57.

    Identification of major cell classes in the developing mammalian nervous system.
    McKay RD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 5.12 (1985 Dec): 3310-28.

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