40.2D6

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SKU: 40.2D6

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Product Name/ID: 40.2D6

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Name: Isl1

Ab Isotype: MIgG1

Gene Symbol:

Antibody Registry ID: AB_528315

Uniprot ID: P61374

RRID:

Entrez Gene ID: 64444

Clonality: Monoclonal

Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .

Clone:

Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)

Myeloma Strain: NS-1

Epitope Mapped: Yes

Antigen Name: Islet-1 homeobox

Epitope Location or Sequence:

Alternate Antigen Name:

Deposit Date: 8/4/1993

Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa

Depositor: Jessell, T.M. / Brenner-Morton, S.

Antigen Sequence:

Depositor Institution: Columbia University / HHMI

Antigen Species: rat

Depositor Notes: Reacted with all tested vertebrate species. The 40.2D6 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.3A4 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: altricial fish larva, Chicken, Fish, Frog, Human, Mouse, Newt, Planaria, Porcine, Rat, Reptile, Vertebrates, Xenopus, Zebrafish

Additional Information:

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Chromatin Immunoprecipitation, FACS, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

40.2D6 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.2D6)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

77 References

Initial Publication

IF References

WB References

IHC References

ChIP References

FFPE References

FACS References

Epitope Map References

Gel Supershift References

All References

Initial Publication

Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
Yamada T
Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

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40.2D6

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