M1/70.15.11.5.2

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SKU: M1/70.15.11.5.2

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Clone ID/Product Name: M1/70.15.11.5.2

Available to For-Profits: Yes

Alternate Antibody Name: M1/70

Gene Symbol: Itgam

Ab Isotype: RIgG2b

Gene Name:

Antibody Registry ID: AB_2234066

Uniprot ID: P05555

RRID:

Entrez Gene ID: 16409

Clonality: Monoclonal

Immunogen: Spleen cells from B10 mice.

Clone:

Immunogen Sequence: Full length protein

Myeloma Strain: NS1

Epitope Mapped: No

Antigen Name: CD11b (Integrin alpha-M, Mac-1 alpha chain), murine

Epitope Location or Sequence:

Alternate Antigen Name:

Deposit Date: 4/4/1989

Antigen Molecular Weight: Predicted: 128 kDa; Apparent: 170 kDa

Depositor: Springer, T.A.

Antigen Sequence:

Depositor Institution: Center for Blood Research, Boston

Antigen Species: Mouse

Depositor Notes: Blocks iC3B rosetting. Binds to granulocytes, monocytes, macrophages, hairy cell leukemia cells, CD5+ human B cells. Doesn't appear to work for western blotting.

Host Species: rat

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Human, Mouse

Additional Information: Mac-1 is a macrophage differenciation antigen and a marker for microglia.

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

M1/70.15.11.5.2 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product M1/70.15.11.5.2)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

24 References

Initial Publication

IF References

IHC References

IP References

FB References

FACS References

All References

Initial Publication

Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
Milstein C
European journal of immunology 8.8 (1978 Aug): 539-51.

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M1/70.15.11.5.2

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