1D4B

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DSHB Data Sheet
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Clone ID/Product Name: 1D4B
Available to For-Profits: Yes
Alternate Antibody Name: CD107a
Gene Symbol: Lamp1
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_528127 
Uniprot ID: P11438 
RRID:  
Entrez Gene ID: 16783 
Clonality: Monoclonal
Immunogen: NIH/3T3 mouse embryo fibroblast tissue culture cell membranes
Clone:
Immunogen Sequence:
Myeloma Strain: P3-NSI/1AG4-1
Epitope Mapped: Yes
Antigen Name: LAMP-1
Epitope Location or Sequence: Lumenal region
Alternate Antigen Name: Lysosomal membrane glycoprotein A, LGP-120, CD107a
Deposit Date: 10/1/1986
Antigen Molecular Weight: Predicted: 41 kDa; Apparent: 110kDa
Depositor: August, J.T.
Antigen Sequence:
Depositor Institution: Johns Hopkins School of Medicine, Pharmacology & Molecular Sciences
Antigen Species: Mouse
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: RRID: AB_528127
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D4B was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product 1D4B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

44 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • All References
    • Initial Publication

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    IF References

    Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes.
    Griffiths G
    The EMBO journal 19.2 (2000 Jan 17): 199-212.

    SseF and SseG are translocated effectors of the type III secretion system of Salmonella pathogenicity island 2 that modulate aggregation of endosomal compartments.
    Hensel M
    Cellular microbiology 4.12 (2002 Dec): 813-24.

    Early intraneuronal Abeta deposition in the hippocampus of APP transgenic mice.
    Jin LW
    Neuroreport 14.1 (2003 Jan 20): 123-9.

    Dynamics of intracellular bacterial replication at the single cell level.
    Holden DW
    Proceedings of the National Academy of Sciences of the United States of America 107.8 (2010 Feb 23): 3746-51.

    Fibrin deposited in the Alzheimer's disease brain promotes neuronal degeneration.
    Strickland S
    Neurobiology of aging 36.2 (2015 Feb): 608-17.

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular & molecular biology research 41.5 (1995): 405-20.

    Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons.
    Sheng ZH
    The Journal of cell biology 217.9 (2018 Sep 3): 3127-3139.

    Impaired Lysosomal Integral Membrane Protein 2-dependent Peroxiredoxin 6 Delivery to Lamellar Bodies Accounts for Altered Alveolar Phospholipid Content in Adaptor Protein-3-deficient pearl Mice.
    Guttentag S
    The Journal of biological chemistry 291.16 (2016 Apr 15): 8414-27.

    Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.
    Dikic I
    eLife 6. (2017 Jun 15): .

    Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis.
    Bovolenta P
    Nature neuroscience 22.8 (2019 Aug): 1258-1268.

    Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts.
    Sheng ZH
    Nature communications 10.1 (2019 Aug 13): 3645.

    Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide.
    Ganley IG
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 621-642.

    Differential Induction of Type I and III Interferons by Staphylococcus aureus.
    Parker D
    Infection and immunity 88.10 (2020 Sep 18): .

    Microglia Do Not Take Up Soluble Amyloid-beta Peptides, But Partially Degrade Them by Secreting Insulin-degrading Enzyme.
    Lemere CA
    Neuroscience 443. (2020 Sep 1): 30-43.

    Complement receptor 3 mediates ruffle-like, actin-rich aggregates during phagocytosis of Leishmania infantum metacyclics.
    Wilson ME
    Experimental parasitology 220. (2021 Jan): 107968.

    Bscl2 Deficiency Does Not Directly Impair the Innate Immune Response in a Murine Model of Generalized Lipodystrophy.
    Rochford JJ
    Journal of clinical medicine 10.3 (2021 Jan 23): .

    The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal.
    Kierbel A
    Scientific reports 11.1 (2021 Jan 27): 2393.

    Epithelial cell-turnover ensures robust coordination of tissue growth in Drosophila ribosomal protein mutants.
    Igaki T
    PLoS genetics 17.1 (2021 Jan): e1009300.

    IRGM1 links mitochondrial quality control to autoimmunity.
    Fessler MB
    Nature immunology 22.3 (2021 Mar): 312-321.

    Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion.
    Rape M
    Developmental cell 56.5 (2021 Mar 8): 588-601.e9.

    Glycogen accumulation in smooth muscle of a Pompe disease mouse model.
    ElMallah MK
    Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi 57.0 (2021): 8-18.

    Genetic Inactivation of Peroxiredoxin-I Impairs the Growth of Human Pancreatic Cancer Cells.
    Assi M
    Antioxidants (Basel, Switzerland) 10.4 (2021 Apr 8): .

    Why adolescent medicine? More illustrative cases.
    Strasburger VC
    Connecticut medicine 49.8 (1985 Aug): 501-3.

    Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes.
    Brumell JH
    Nature communications 12.1 (2021 Aug 17): 4999.

    WB References

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.
    Haas A
    Molecular biology of the cell 29.4 (2018 Feb 15): 452-465.

    Impaired Lysosomal Integral Membrane Protein 2-dependent Peroxiredoxin 6 Delivery to Lamellar Bodies Accounts for Altered Alveolar Phospholipid Content in Adaptor Protein-3-deficient pearl Mice.
    Guttentag S
    The Journal of biological chemistry 291.16 (2016 Apr 15): 8414-27.

    Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
    Pichaud F
    Development (Cambridge, England) 146.15 (2019 Aug 12): .

    Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition.
    Matthews QL
    Pathogens (Basel, Switzerland) 8.4 (2019 Dec 14): .

    IHC References

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide.
    Ganley IG
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 621-642.

    Microglia Do Not Take Up Soluble Amyloid-beta Peptides, But Partially Degrade Them by Secreting Insulin-degrading Enzyme.
    Lemere CA
    Neuroscience 443. (2020 Sep 1): 30-43.

    Loss of Furin in β-Cells Induces an mTORC1-ATF4 Anabolic Pathway That Leads to β-Cell Dysfunction.
    Creemers JWM
    Diabetes 70.2 (2021 Feb): 492-503.

    ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H(+)-ATPases is essential for brain development in humans and mice.
    Saitsu H
    Nature communications 12.1 (2021 Apr 8): 2107.

    SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo.
    Hornef MW
    Gut microbes 13.1 (2021 Jan-Dec): 1973836.

    IP References

    Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins.
    Mellman I
    Proceedings of the National Academy of Sciences of the United States of America 85.20 (1988 Oct): 7577-81.

    Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells.
    August JT
    The Journal of biological chemistry 256.13 (1981 Jul 10): 7023-7.

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular & molecular biology research 41.5 (1995): 405-20.

    ELISA References

    Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition.
    Matthews QL
    Pathogens (Basel, Switzerland) 8.4 (2019 Dec 14): .

    FFPE References

    SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo.
    Hornef MW
    Gut microbes 13.1 (2021 Jan-Dec): 1973836.

    All References

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide.
    Ganley IG
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 621-642.

    Microglia Do Not Take Up Soluble Amyloid-beta Peptides, But Partially Degrade Them by Secreting Insulin-degrading Enzyme.
    Lemere CA
    Neuroscience 443. (2020 Sep 1): 30-43.

    Loss of Furin in β-Cells Induces an mTORC1-ATF4 Anabolic Pathway That Leads to β-Cell Dysfunction.
    Creemers JWM
    Diabetes 70.2 (2021 Feb): 492-503.

    ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H(+)-ATPases is essential for brain development in humans and mice.
    Saitsu H
    Nature communications 12.1 (2021 Apr 8): 2107.

    SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo.
    Hornef MW
    Gut microbes 13.1 (2021 Jan-Dec): 1973836.

    Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes.
    Griffiths G
    The EMBO journal 19.2 (2000 Jan 17): 199-212.

    SseF and SseG are translocated effectors of the type III secretion system of Salmonella pathogenicity island 2 that modulate aggregation of endosomal compartments.
    Hensel M
    Cellular microbiology 4.12 (2002 Dec): 813-24.

    Early intraneuronal Abeta deposition in the hippocampus of APP transgenic mice.
    Jin LW
    Neuroreport 14.1 (2003 Jan 20): 123-9.

    Dynamics of intracellular bacterial replication at the single cell level.
    Holden DW
    Proceedings of the National Academy of Sciences of the United States of America 107.8 (2010 Feb 23): 3746-51.

    Fibrin deposited in the Alzheimer's disease brain promotes neuronal degeneration.
    Strickland S
    Neurobiology of aging 36.2 (2015 Feb): 608-17.

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular & molecular biology research 41.5 (1995): 405-20.

    Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons.
    Sheng ZH
    The Journal of cell biology 217.9 (2018 Sep 3): 3127-3139.

    Impaired Lysosomal Integral Membrane Protein 2-dependent Peroxiredoxin 6 Delivery to Lamellar Bodies Accounts for Altered Alveolar Phospholipid Content in Adaptor Protein-3-deficient pearl Mice.
    Guttentag S
    The Journal of biological chemistry 291.16 (2016 Apr 15): 8414-27.

    Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.
    Dikic I
    eLife 6. (2017 Jun 15): .

    Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis.
    Bovolenta P
    Nature neuroscience 22.8 (2019 Aug): 1258-1268.

    Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts.
    Sheng ZH
    Nature communications 10.1 (2019 Aug 13): 3645.

    Differential Induction of Type I and III Interferons by Staphylococcus aureus.
    Parker D
    Infection and immunity 88.10 (2020 Sep 18): .

    Complement receptor 3 mediates ruffle-like, actin-rich aggregates during phagocytosis of Leishmania infantum metacyclics.
    Wilson ME
    Experimental parasitology 220. (2021 Jan): 107968.

    Bscl2 Deficiency Does Not Directly Impair the Innate Immune Response in a Murine Model of Generalized Lipodystrophy.
    Rochford JJ
    Journal of clinical medicine 10.3 (2021 Jan 23): .

    The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal.
    Kierbel A
    Scientific reports 11.1 (2021 Jan 27): 2393.

    Epithelial cell-turnover ensures robust coordination of tissue growth in Drosophila ribosomal protein mutants.
    Igaki T
    PLoS genetics 17.1 (2021 Jan): e1009300.

    IRGM1 links mitochondrial quality control to autoimmunity.
    Fessler MB
    Nature immunology 22.3 (2021 Mar): 312-321.

    Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion.
    Rape M
    Developmental cell 56.5 (2021 Mar 8): 588-601.e9.

    Glycogen accumulation in smooth muscle of a Pompe disease mouse model.
    ElMallah MK
    Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi 57.0 (2021): 8-18.

    Genetic Inactivation of Peroxiredoxin-I Impairs the Growth of Human Pancreatic Cancer Cells.
    Assi M
    Antioxidants (Basel, Switzerland) 10.4 (2021 Apr 8): .

    Why adolescent medicine? More illustrative cases.
    Strasburger VC
    Connecticut medicine 49.8 (1985 Aug): 501-3.

    Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes.
    Brumell JH
    Nature communications 12.1 (2021 Aug 17): 4999.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.
    Haas A
    Molecular biology of the cell 29.4 (2018 Feb 15): 452-465.

    Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
    Pichaud F
    Development (Cambridge, England) 146.15 (2019 Aug 12): .

    Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition.
    Matthews QL
    Pathogens (Basel, Switzerland) 8.4 (2019 Dec 14): .

    Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins.
    Mellman I
    Proceedings of the National Academy of Sciences of the United States of America 85.20 (1988 Oct): 7577-81.

    Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells.
    August JT
    The Journal of biological chemistry 256.13 (1981 Jul 10): 7023-7.

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    Lysosomal cathepsin B participates in the podosome-mediated extracellular matrix degradation and invasion via secreted lysosomes in v-Src fibroblasts.
    Band H
    Cancer research 68.22 (2008 Nov 15): 9147-56.

    Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron.
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    Endosomal-sorting complexes required for transport (ESCRT) pathway-dependent endosomal traffic regulates the localization of active Src at focal adhesions.
    Band H
    Proceedings of the National Academy of Sciences of the United States of America 107.37 (2010 Sep 14): 16107-12.

    alpha6beta1-Integrin, a major cell surface carrier of beta1-6-branched oligosaccharides, mediates migration of EJ-ras-transformed fibroblasts on laminin-1 independently of its glycosylation state.
    Brentani RR
    Cancer research 56.7 (1996 Apr 1): 1682-9.

    A kinetic analysis of biosynthesis and localization of a lysosome-associated membrane glycoprotein.
    August JT
    Archives of biochemistry and biophysics 249.2 (1986 Sep): 522-32.

    Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2.
    August JT
    Biochemical Society symposium 51. (1986): 97-112.

    Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells.
    August JT
    Archives of biochemistry and biophysics 239.2 (1985 Jun): 574-86.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Murine cell surface glycoproteins. Identification, purification, and characterization of a major glycosylated component of 110,000 daltons by use of a monoclonal antibody.
    August JT
    The Journal of biological chemistry 257.7 (1982 Apr 10): 3970-7.

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