12G10 anti-alpha-tubulin

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SKU: 12G10 anti-alpha-tubulin

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 12G10 anti-alpha-tubulin
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ATU1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_1157911 
Uniprot ID: P41351 
RRID:  
Entrez Gene ID: 7838142 
Clonality: Monoclonal
Immunogen: alpha tubulin, Tetrahymena thermophila and Tetrahymena pyriformis (mixture)
Clone:
Immunogen Sequence: alpha tubulin
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: tubulin (alpha-)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/21/2001
Antigen Molecular Weight: Predicted: 49.59 kDa
Depositor: Frankel, J. / Nelsen, E.M.
Antigen Sequence: 3139885 8020112
Depositor Institution: University of Iowa
Antigen Species: Tetrahymena
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Algae, Avian, C. elegans, Cephalopod, Dictyostelium, Drosophila, Fish, Hermit Crab, Human, Medusa, Mosquito, Mouse, Protozoa, Quail, Saccharomyces, Tetrahymena, Toxoplasma gondii, Xenopus
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12G10 anti-alpha-tubulin was deposited to the DSHB by Frankel, J. / Nelsen, E.M. (DSHB Hybridoma Product 12G10 anti-alpha-tubulin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

43 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Hyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell.
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    [Digestive tract involvement in collagenoses].
    Manolescu N
    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna 39.6 (1987 Nov-Dec): 513-20.

    Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro.
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    A family of protein-deglutamylating enzymes associated with neurodegeneration.
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    Evolution of early embryogenesis in rhabditid nematodes.
    Piano F
    Developmental biology 335.1 (2009 Nov 1): 253-62.

    α-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium.
    Weis WI
    Developmental cell 23.3 (2012 Sep 11): 533-46.

    Muscle and nerve net organization in stalked jellyfish (Medusozoa: Staurozoa).
    Page LR
    Journal of morphology 278.1 (2017 Jan): 29-49.

    Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods.
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods.
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons.
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Microtubule glycylation promotes attachment of basal bodies to the cell cortex.
    Pearson CG
    Journal of cell science 132.15 (2019 Aug 7): .

    3β, 6β-dichloro-5-hydroxy-5α-cholestane facilitates neuronal development through modulating TrkA signaling regulated proteins in primary hippocampal neuron.
    Moon IS
    Scientific reports 9.1 (2019 Dec 12): 18919.

    Analysis of the "centrosome-ome" identifies MCPH1 deletion as a cause of centrosome amplification in human cancer.
    Burkard ME
    Scientific reports 10.1 (2020 Jul 17): 11921.

    Centella asiatica promotes early differentiation, axodendritic maturation and synaptic formation in primary hippocampal neurons.
    Moon IS
    Neurochemistry international 144. (2021 Mar): 104957.

    Whole-Mount RNA In Situ Hybridization and Immunofluorescence of Xenopus Embryos and Tadpoles.
    Willsey HR
    Cold Spring Harbor protocols 2021.10 (2021 Oct 1): pdb.prot105635.

    Composition and function of the C1b/C1f region in the ciliary central apparatus.
    Wloga D
    Scientific reports 11.1 (2021 Jun 3): 11760.

    Polyglycylation domain of beta-tubulin maintains axonemal architecture and affects cytokinesis in Tetrahymena.
    Gaertig J
    Nature cell biology 4.3 (2002 Mar): 256-9.

    WB References

    Muscle biopsies off-set normal cellular signaling in surrounding musculature.
    Vissing J
    Neuromuscular disorders : NMD 23.12 (2013 Dec): 981-5.

    [Darker side of tanning from artificial radiation source].
    David M
    Harefuah 117.9 (1989 Nov 1): 273-4.

    In situ visualization of protein interactions in sensory neurons: glutamic acid-rich proteins (GARPs) play differential roles for photoreceptor outer segment scaffolding.
    Goldberg AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.31 (2011 Aug 3): 11231-43.

    Extramitochondrial Assembly of Mitochondrial Targeting Signal Disrupted Mitochondrial Enzyme Aldehyde Dehydrogenase.
    Noree C
    Scientific reports 8.1 (2018 Apr 18): 6186.

    COMMD1-mediated ubiquitination regulates CFTR trafficking.
    Fanen P
    PloS one 6.3 (2011 Mar 31): e18334.

    Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction.
    Nose A
    Biology open 1.4 (2012 Apr 15): 362-75.

    SIRT6 protects against pathological damage caused by diet-induced obesity.
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
    Pradet-Balade B
    The Journal of biological chemistry 289.9 (2014 Feb 28): 6236-47.

    Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation.
    Janke C
    Cell 137.6 (2009 Jun 12): 1076-87.

    Diallyl disulfide inhibits ethanol-induced pulmonary cell vitamin D and antimicrobial peptide cathelicidin depletion.
    McCaskill M
    Alcohol (Fayetteville, N.Y.) 80. (2019 Nov): 99-108.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    Listeria Membrane Protrusion Collapse: Requirement of Cyclophilin A for Listeria Cell-to-Cell Spreading.
    Guttman JA
    The Journal of infectious diseases 219.1 (2019 Jan 1): 145-153.

    Contribution of extracellular O-GlcNAc to the stability of folded epidermal growth factor-like domains and Notch1 trafficking.
    Okajima T
    Biochemical and biophysical research communications 526.1 (2020 May 21): 184-190.

    Analysis of the "centrosome-ome" identifies MCPH1 deletion as a cause of centrosome amplification in human cancer.
    Burkard ME
    Scientific reports 10.1 (2020 Jul 17): 11921.

    Predominant and novel de novo variants in 29 individuals with ALG13 deficiency: Clinical description, biomarker status, biochemical analysis, and treatment suggestions.
    Freeze HH
    Journal of inherited metabolic disease 43.6 (2020 Nov): 1333-1348.

    Age-related changes in isolated mouse skeletal muscle function are dependent on sex, muscle, and contractility mode.
    Tallis J
    American journal of physiology. Regulatory, integrative and comparative physiology 319.3 (2020 Sep 1): R296-R314.



    . (): .

    Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis.
    West AP
    Journal of immunology (Baltimore, Md. : 1950) 206.8 (2021 Apr 15): 1890-1900.

    Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.
    Zimmers TA
    The Journal of experimental medicine 218.6 (2021 Jun 7): .

    Activation of Anopheles stephensi Pantothenate Kinase and Coenzyme A Biosynthesis Reduces Infection with Diverse Plasmodium Species in the Mosquito Host.
    Luckhart S
    Biomolecules 11.6 (2021 May 29): .

    Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster.
    Leal MC
    The European journal of neuroscience 54.6 (2021 Sep): 5915-5931.

    Targeted disruption of PKC from AKAP signaling complexes.
    Kennedy EJ
    RSC chemical biology 2.4 (2021 Aug 5): 1227-1231.

    IHC References
    FFPE References
    All References

    Tubulin glutamylation regulates ciliary motility by altering inner dynein arm activity.
    Gaertig J
    Current biology : CB 20.5 (2010 Mar 9): 435-40.

    Hyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell.
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro.
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    A brown alga Sargassum fulvellum facilitates neuronal maturation and synaptogenesis.
    Moon IS
    In vitro cellular & developmental biology. Animal 48.8 (2012 Sep): 535-44.

    A family of protein-deglutamylating enzymes associated with neurodegeneration.
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    [Digestive tract involvement in collagenoses].
    Manolescu N
    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna 39.6 (1987 Nov-Dec): 513-20.

    Evolution of early embryogenesis in rhabditid nematodes.
    Piano F
    Developmental biology 335.1 (2009 Nov 1): 253-62.

    α-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium.
    Weis WI
    Developmental cell 23.3 (2012 Sep 11): 533-46.

    Muscle and nerve net organization in stalked jellyfish (Medusozoa: Staurozoa).
    Page LR
    Journal of morphology 278.1 (2017 Jan): 29-49.

    Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods.
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons.
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Microtubule glycylation promotes attachment of basal bodies to the cell cortex.
    Pearson CG
    Journal of cell science 132.15 (2019 Aug 7): .

    3β, 6β-dichloro-5-hydroxy-5α-cholestane facilitates neuronal development through modulating TrkA signaling regulated proteins in primary hippocampal neuron.
    Moon IS
    Scientific reports 9.1 (2019 Dec 12): 18919.

    Analysis of the "centrosome-ome" identifies MCPH1 deletion as a cause of centrosome amplification in human cancer.
    Burkard ME
    Scientific reports 10.1 (2020 Jul 17): 11921.

    Centella asiatica promotes early differentiation, axodendritic maturation and synaptic formation in primary hippocampal neurons.
    Moon IS
    Neurochemistry international 144. (2021 Mar): 104957.

    Whole-Mount RNA In Situ Hybridization and Immunofluorescence of Xenopus Embryos and Tadpoles.
    Willsey HR
    Cold Spring Harbor protocols 2021.10 (2021 Oct 1): pdb.prot105635.

    Composition and function of the C1b/C1f region in the ciliary central apparatus.
    Wloga D
    Scientific reports 11.1 (2021 Jun 3): 11760.

    Polyglycylation domain of beta-tubulin maintains axonemal architecture and affects cytokinesis in Tetrahymena.
    Gaertig J
    Nature cell biology 4.3 (2002 Mar): 256-9.

    Muscle biopsies off-set normal cellular signaling in surrounding musculature.
    Vissing J
    Neuromuscular disorders : NMD 23.12 (2013 Dec): 981-5.

    [Darker side of tanning from artificial radiation source].
    David M
    Harefuah 117.9 (1989 Nov 1): 273-4.

    In situ visualization of protein interactions in sensory neurons: glutamic acid-rich proteins (GARPs) play differential roles for photoreceptor outer segment scaffolding.
    Goldberg AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.31 (2011 Aug 3): 11231-43.

    Extramitochondrial Assembly of Mitochondrial Targeting Signal Disrupted Mitochondrial Enzyme Aldehyde Dehydrogenase.
    Noree C
    Scientific reports 8.1 (2018 Apr 18): 6186.

    COMMD1-mediated ubiquitination regulates CFTR trafficking.
    Fanen P
    PloS one 6.3 (2011 Mar 31): e18334.

    Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction.
    Nose A
    Biology open 1.4 (2012 Apr 15): 362-75.

    SIRT6 protects against pathological damage caused by diet-induced obesity.
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
    Pradet-Balade B
    The Journal of biological chemistry 289.9 (2014 Feb 28): 6236-47.

    Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation.
    Janke C
    Cell 137.6 (2009 Jun 12): 1076-87.

    Diallyl disulfide inhibits ethanol-induced pulmonary cell vitamin D and antimicrobial peptide cathelicidin depletion.
    McCaskill M
    Alcohol (Fayetteville, N.Y.) 80. (2019 Nov): 99-108.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    Listeria Membrane Protrusion Collapse: Requirement of Cyclophilin A for Listeria Cell-to-Cell Spreading.
    Guttman JA
    The Journal of infectious diseases 219.1 (2019 Jan 1): 145-153.

    Contribution of extracellular O-GlcNAc to the stability of folded epidermal growth factor-like domains and Notch1 trafficking.
    Okajima T
    Biochemical and biophysical research communications 526.1 (2020 May 21): 184-190.

    Predominant and novel de novo variants in 29 individuals with ALG13 deficiency: Clinical description, biomarker status, biochemical analysis, and treatment suggestions.
    Freeze HH
    Journal of inherited metabolic disease 43.6 (2020 Nov): 1333-1348.

    Age-related changes in isolated mouse skeletal muscle function are dependent on sex, muscle, and contractility mode.
    Tallis J
    American journal of physiology. Regulatory, integrative and comparative physiology 319.3 (2020 Sep 1): R296-R314.



    . (): .

    Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis.
    West AP
    Journal of immunology (Baltimore, Md. : 1950) 206.8 (2021 Apr 15): 1890-1900.

    Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.
    Zimmers TA
    The Journal of experimental medicine 218.6 (2021 Jun 7): .

    Activation of Anopheles stephensi Pantothenate Kinase and Coenzyme A Biosynthesis Reduces Infection with Diverse Plasmodium Species in the Mosquito Host.
    Luckhart S
    Biomolecules 11.6 (2021 May 29): .

    Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster.
    Leal MC
    The European journal of neuroscience 54.6 (2021 Sep): 5915-5931.

    Targeted disruption of PKC from AKAP signaling complexes.
    Kennedy EJ
    RSC chemical biology 2.4 (2021 Aug 5): 1227-1231.

    Fine structural organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus.
    Wolff G
    The Journal of comparative neurology 520.13 (2012 Sep 1): 2847-63.

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Cellular polarity in ciliates: persistence of global polarity in a disorganized mutant of Tetrahymena thermophila that disrupts cytoskeletal organization.
    Frankel J
    Developmental biology 169.2 (1995 Jun): 644-61.

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