9 D10

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SKU: 9 D10

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 9 D10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TTN
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_528491 
Uniprot ID: F1N757 
RRID:  
Entrez Gene ID: 540561 
Clonality: Monoclonal
Immunogen: Titin (purified) bovine heart.
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: Titin
Epitope Location or Sequence: proline-glutamate-valine-lysine (PEVK) domain;
Alternate Antigen Name:
Deposit Date: 4/13/1989
Antigen Molecular Weight: 3000-4000 kDa
Depositor: Greaser, M.L.
Antigen Sequence:
Depositor Institution: University of Wisconsin-Madison
Antigen Species: Bovine
Depositor Notes: Stains 4% paraformaldehyde or methanol fixed cells and paraffin sections; also 0.1% glutaraldehyde
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Canine, Chicken, Drosophila, Fish, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Xenopus
Additional Information: single titin molecule labeling PMID: 24357719; immunoelectron microscopy PMID: 9724620 9277364 9172076
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9 D10 was deposited to the DSHB by Greaser, M.L. (DSHB Hybridoma Product 9 D10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

37 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.
    Greaser ML
    Journal of muscle research and cell motility 6.3 (1985 Jun): 293-312.

    IF References

    Left ventricular torsional mechanics and myocardial iron load in beta-thalassaemia major: a potential role of titin degradation.
    Cheung YF
    BMC cardiovascular disorders 14. (2014 Apr 12): 49.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Heterogeneity of myotubes generated by the MyoD and E12 basic helix-loop-helix transcription factors in otherwise non-differentiation growth conditions.
    Parpura V
    Biomaterials 35.7 (2014 Feb): 2188-98.

    Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.
    Gregorio CC
    Methods (San Diego, Calif.) 66.3 (2014 Apr 1): 370-9.

    Critical roles for multiple formins during cardiac myofibril development and repair.
    Goode BL
    Molecular biology of the cell 25.6 (2014 Mar): 811-27.

    DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.
    Mihály J
    PLoS genetics 10.2 (2014 Feb): e1004166.

    Epicardium is required for sarcomeric maturation and cardiomyocyte growth in the ventricular compact layer mediated by transforming growth factor β and fibroblast growth factor before the onset of coronary circulation.
    Nakajima Y
    Congenital anomalies 54.3 (2014 Aug): 162-71.

    Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury.
    Schulz R
    Circulation 122.20 (2010 Nov 16): 2039-47.

    Pathways of Ca²⁺ entry and cytoskeletal damage following eccentric contractions in mouse skeletal muscle.
    Allen DG
    Journal of applied physiology (Bethesda, Md. : 1985) 112.12 (2012 Jun): 2077-86.

    Comparison of cardiomyogenic potential among human ESC and iPSC lines.
    Lough JW
    Cell transplantation 21.11 (2012): 2523-30.

    Transcription factor rational design improves directed differentiation of human mesenchymal stem cells into skeletal myocytes.
    de Vries AA
    Molecular therapy : the journal of the American Society of Gene Therapy 19.7 (2011 Jul): 1331-41.

    Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload.
    Ross RS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4252-64.

    A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation.
    Martin U
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 463-73.

    Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules.
    Sanger JW
    Cytoskeleton (Hoboken, N.J.) 67.12 (2010 Dec): 808-23.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Sequence and mechanical implications of titin's PEVK region.
    Kumazawa Y
    Advances in experimental medicine and biology 481. (2000): 53-63; discussion 64-6, 107-10.

    Inhibition of nebulin and connectin (titin) for assembly of actin filaments during myofibrillogenesis.
    Shimada Y
    Tissue & cell 32.3 (2000 Jun): 223-7.

    Developmental relationship of myosin binding proteins (myomesin, connectin and C-protein) to myosin in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell structure and function 25.3 (2000 Jun): 177-85.

    Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells.
    Shimada Y
    Experimental cell research 252.1 (1999 Oct 10): 33-40.

    Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts.
    Field LJ
    The Journal of clinical investigation 98.1 (1996 Jul 1): 216-24.

    Skeletal muscle myofibrillogenesis as revealed with a monoclonal antibody to titin in combination with detection of the alpha- and gamma-isoforms of actin.
    Lessard JL
    Developmental biology 132.1 (1989 Mar): 35-44.

    Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin.
    Lessard JL
    The Journal of cell biology 107.3 (1988 Sep): 1075-83.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    RYBP is important for cardiac progenitor cell development and sarcomere formation.
    Pirity MK
    PloS one 15.7 (2020): e0235922.

    WB References
    IHC References
    Epitope Map References

    PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10.
    Granzier H
    Journal of structural biology 122.1-2 (1998): 188-96.

    Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.
    Greaser ML
    Journal of muscle research and cell motility 6.3 (1985 Jun): 293-312.

    Sequence and mechanical implications of titin's PEVK region.
    Kumazawa Y
    Advances in experimental medicine and biology 481. (2000): 53-63; discussion 64-6, 107-10.

    Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury.
    Schulz R
    Circulation 122.20 (2010 Nov 16): 2039-47.

    All References

    Left ventricular torsional mechanics and myocardial iron load in beta-thalassaemia major: a potential role of titin degradation.
    Cheung YF
    BMC cardiovascular disorders 14. (2014 Apr 12): 49.

    Heterogeneity of myotubes generated by the MyoD and E12 basic helix-loop-helix transcription factors in otherwise non-differentiation growth conditions.
    Parpura V
    Biomaterials 35.7 (2014 Feb): 2188-98.

    Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury.
    Schulz R
    Circulation 122.20 (2010 Nov 16): 2039-47.

    Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.
    Greaser ML
    Journal of muscle research and cell motility 6.3 (1985 Jun): 293-312.

    Arginylation regulates myofibrils to maintain heart function and prevent dilated cardiomyopathy.
    Kashina A
    Journal of molecular and cellular cardiology 53.3 (2012 Sep): 333-41.

    Rearrangement of intercellular junctions and cytoskeletal proteins during rabbit myocardium development.
    Viebahn C
    European journal of cell biology 68.1 (1995 Sep): 62-9.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.
    Gregorio CC
    Methods (San Diego, Calif.) 66.3 (2014 Apr 1): 370-9.

    Critical roles for multiple formins during cardiac myofibril development and repair.
    Goode BL
    Molecular biology of the cell 25.6 (2014 Mar): 811-27.

    DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.
    Mihály J
    PLoS genetics 10.2 (2014 Feb): e1004166.

    Epicardium is required for sarcomeric maturation and cardiomyocyte growth in the ventricular compact layer mediated by transforming growth factor β and fibroblast growth factor before the onset of coronary circulation.
    Nakajima Y
    Congenital anomalies 54.3 (2014 Aug): 162-71.

    Pathways of Ca²⁺ entry and cytoskeletal damage following eccentric contractions in mouse skeletal muscle.
    Allen DG
    Journal of applied physiology (Bethesda, Md. : 1985) 112.12 (2012 Jun): 2077-86.

    Comparison of cardiomyogenic potential among human ESC and iPSC lines.
    Lough JW
    Cell transplantation 21.11 (2012): 2523-30.

    Transcription factor rational design improves directed differentiation of human mesenchymal stem cells into skeletal myocytes.
    de Vries AA
    Molecular therapy : the journal of the American Society of Gene Therapy 19.7 (2011 Jul): 1331-41.

    Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload.
    Ross RS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4252-64.

    A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation.
    Martin U
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 463-73.

    Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules.
    Sanger JW
    Cytoskeleton (Hoboken, N.J.) 67.12 (2010 Dec): 808-23.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Sequence and mechanical implications of titin's PEVK region.
    Kumazawa Y
    Advances in experimental medicine and biology 481. (2000): 53-63; discussion 64-6, 107-10.

    Inhibition of nebulin and connectin (titin) for assembly of actin filaments during myofibrillogenesis.
    Shimada Y
    Tissue & cell 32.3 (2000 Jun): 223-7.

    Developmental relationship of myosin binding proteins (myomesin, connectin and C-protein) to myosin in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell structure and function 25.3 (2000 Jun): 177-85.

    Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells.
    Shimada Y
    Experimental cell research 252.1 (1999 Oct 10): 33-40.

    Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts.
    Field LJ
    The Journal of clinical investigation 98.1 (1996 Jul 1): 216-24.

    Skeletal muscle myofibrillogenesis as revealed with a monoclonal antibody to titin in combination with detection of the alpha- and gamma-isoforms of actin.
    Lessard JL
    Developmental biology 132.1 (1989 Mar): 35-44.

    Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin.
    Lessard JL
    The Journal of cell biology 107.3 (1988 Sep): 1075-83.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    RYBP is important for cardiac progenitor cell development and sarcomere formation.
    Pirity MK
    PloS one 15.7 (2020): e0235922.

    S-glutathionylation of cryptic cysteines enhances titin elasticity by blocking protein folding.
    Fernández JM
    Cell 156.6 (2014 Mar 13): 1235-1246.

    Titin-based stiffening of muscle fibers in Ehlers-Danlos Syndrome.
    Granzier H
    Journal of applied physiology (Bethesda, Md. : 1985) 112.7 (2012 Apr): 1157-65.

    [Expression of titin in the myocardium of spontaneously hypertensive rats during in the course of hypertrophy].
    Podlubnaia ZA
    Biofizika 55.4 (2010 Jul-Aug): 612-8.

    Fast skeletal muscle troponin activation increases force of mouse fast skeletal muscle and ameliorates weakness due to nebulin-deficiency.
    Granzier H
    PloS one 8.2 (2013): e55861.

    PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10.
    Granzier H
    Journal of structural biology 122.1-2 (1998): 188-96.

    Development of connectin/titin and nebulin in striated muscles of chicken.
    Maruyama K
    Advances in biophysics 33. (1996): 223-33.

    Cultured pig rhabdomyosarcoma cells with a deletion of the Xq24-qter chromosome region: an immunochemical and cytogenetic characterization.
    Ramaekers FC
    American journal of veterinary research 56.8 (1995 Aug): 1062-9.

    Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins.
    Ramaekers FC
    Differentiation; research in biological diversity 46.2 (1991 Mar): 105-15.

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