F59

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SKU: F59

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Clone ID/Product Name: F59

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: MYH1A

Ab Isotype: MIgG1, kappa light chain

Gene Name:

Antibody Registry ID: AB_528373

Uniprot ID: Q91352

RRID:

Entrez Gene ID: 417309

Clonality: Monoclonal

Immunogen: Myosin isolated from pectoralis major

Clone:

Immunogen Sequence: Full length protein

Myeloma Strain: P3/NS1.1Ag4-1

Epitope Mapped: Yes

Antigen Name: Myosin heavy chain (all fast isoforms)

Epitope Location or Sequence: a.a. 200-250

Alternate Antigen Name:

Deposit Date: 2/12/2004

Antigen Molecular Weight: ~223 kDa

Depositor: Stockdale, F.E.

Antigen Sequence:

Depositor Institution: Stanford University Medical Center

Antigen Species: Chicken

Depositor Notes: This antibody is specific for myosin heavy chain in fast contracting skeletal muscle and atrial and ventricular myosin heavy chains.

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Amphibian, Avian, Canine, Fish, Human, Mouse, Quail, Rabbit, Rat, Shark, Turtle, Xenopus, Zebrafish

Additional Information:

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

F59 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F59)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

41 References

Initial Publication

IF References

WB References

IHC References

IP References

Epitope Map References

All References

Initial Publication

Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
Stockdale FE
The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

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F59

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