BA-D5

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SKU: BA-D5

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: BA-D5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MYH7
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2235587 
Uniprot ID: Q9BE40 
RRID:  
Entrez Gene ID: 281337 
Clonality: Monoclonal
Immunogen: Native myosin preparations
Clone:
Immunogen Sequence: Full length native protein
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Myosin heavy chain Type I
Epitope Location or Sequence:
Alternate Antigen Name: Myosin heavy chain 1
Deposit Date: 3/25/2009
Antigen Molecular Weight: 222.9 kDa
Depositor: Schiaffino, S.
Antigen Sequence:
Depositor Institution: Universita degli Studi di Padova
Antigen Species: Bovine
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Canine, Fish, Goat, Guinea Pig, Horse, Human, Lamb, Llama, Mouse, Porcine, Rabbit, Rat, Zebrafish
Additional Information: Muscle contraction. RRID:AB_2235587
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BA-D5 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BA-D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

43 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
    • Initial Publication

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    IF References

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis.
    Sahenk Z
    Skeletal muscle 7.1 (2017 Dec 14): 27.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.
    McClung JM
    JCI insight 3.21 (2018 Nov 2): .

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging.
    Mann M
    Cell reports 19.11 (2017 Jun 13): 2396-2409.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading.
    Dupont-Versteegden EE
    GeroScience 43.1 (2021 Feb): 65-83.

    Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice.
    Rovere-Querini P
    Aging 12.23 (2020 Nov 26): 23497-23508.

    A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy.
    Zhang ZJ
    Acta neuropathologica communications 9.1 (2021 Feb 8): 23.

    Longitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy.
    Höke A
    Scientific data 6.1 (2019 Sep 24): 179.

    Twenty-one days of low-intensity eccentric training improve morphological characteristics and function of soleus muscles of mdx mice.
    Cornachione AS
    Scientific reports 11.1 (2021 Feb 11): 3579.

    Pioglitazone does not synergize with mirabegron to increase beige fat or further improve glucose metabolism.
    Kern PA
    JCI insight 6.6 (2021 Mar 22): .

    Single-leg exercise training augments in vivo skeletal muscle oxidative flux and vascular content and function in adults with type 2 diabetes.
    Regensteiner JG
    The Journal of physiology 600.4 (2022 Feb): 963-978.

    The ROS scavenger PDTC affects adaptation to treadmill running in mice: distinct effects on murine body mass, resting heart rate and skeletal muscle fiber type composition.
    Munz B
    The Journal of experimental biology 224.Pt 6 (2021 Mar 18): .

    Higher strength gain after hypoxic vs normoxic resistance training despite no changes in muscle thickness and fractional protein synthetic rate.
    Deldicque L
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.8 (2021 Aug): e21773.

    WB References

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    β-Cryptoxanthin Improves p62 Accumulation and Muscle Atrophy in the Soleus Muscle of Senescence-Accelerated Mouse-Prone 1 Mice.
    Yamaji R
    Nutrients 12.8 (2020 Jul 22): .

    Myosin heavy chain isoform and metabolic profile differ in beef steaks varying in tenderness.
    Matarneh SK
    Meat science 170. (2020 Dec): 108266.

    A maternal high-fat/low-fiber diet impairs glucose tolerance and induces the formation of glycolytic muscle fibers in neonatal offspring.
    Tan C
    European journal of nutrition 60.5 (2021 Aug): 2709-2718.

    MicroRNA-129-5p inhibits C2C12 myogenesis and represses slow fiber gene expression in vitro.
    Li X
    American journal of physiology. Cell physiology 320.6 (2021 Jun 1): C1031-C1041.

    Higher strength gain after hypoxic vs normoxic resistance training despite no changes in muscle thickness and fractional protein synthetic rate.
    Deldicque L
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.8 (2021 Aug): e21773.

    IHC References

    New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes.
    Rivero JL
    Cell and tissue research 323.2 (2006 Feb): 283-303.

    Sulf1 modulates BMP signaling and is required for somite morphogenesis and development of the horizontal myoseptum.
    Pownall ME
    Developmental biology 378.2 (2013 Jun 15): 107-21.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study.
    Peterson CA
    Journal of translational medicine 14.1 (2016 Sep 29): 284.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Cellular and morphological changes with EAA supplementation before and after total knee arthroplasty.
    Dreyer HC
    Journal of applied physiology (Bethesda, Md. : 1985) 127.2 (2019 Aug 1): 531-545.

    Hypertrophic muscle growth and metabolic efficiency were impaired by chronic heat stress, improved by zilpaterol supplementation, and not affected by ractopamine supplementation in feedlot lambs1.
    Yates DT
    Journal of animal science 97.10 (2019 Oct 3): 4101-4113.

    Skeletal muscle transcriptional networks linked to type I myofiber grouping in Parkinson's disease.
    Bamman MM
    Journal of applied physiology (Bethesda, Md. : 1985) 128.2 (2020 Feb 1): 229-240.

    Countering disuse atrophy in older adults with low-volume leucine supplementation.
    Paddon-Jones D
    Journal of applied physiology (Bethesda, Md. : 1985) 128.4 (2020 Apr 1): 967-977.

    Regular endurance exercise of overloaded muscle of young and old male mice does not attenuate hypertrophy and improves fatigue resistance.
    Degens H
    GeroScience 43.2 (2021 Apr): 741-757.

    Exercise-mediated alteration of hippocampal Dicer mRNA and miRNAs is associated with lower BACE1 gene expression and Aβ(1-42) in female 3xTg-AD mice.
    Peterson CA
    Journal of neurophysiology 124.6 (2020 Dec 1): 1571-1577.

    IGF-1 infusion to fetal sheep increases organ growth but not by stimulating nutrient transfer to the fetus.
    Brown LD
    American journal of physiology. Endocrinology and metabolism 320.3 (2021 Mar 1): E527-E538.

    Mss51 deletion increases endurance and ameliorates histopathology in the mdx mouse model of Duchenne muscular dystrophy.
    Wagner KR
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.2 (2021 Feb): e21276.

    Single-leg exercise training augments in vivo skeletal muscle oxidative flux and vascular content and function in adults with type 2 diabetes.
    Regensteiner JG
    The Journal of physiology 600.4 (2022 Feb): 963-978.

    Home apnea monitoring.
    Hutchins KH
    Pediatric nursing 12.4 (1986 Jul-Aug): 259-62, 304.

    Reduced Physical Activity Alters the Leucine-Stimulated Translatome in Aged Skeletal Muscle.
    Drummond MJ
    The journals of gerontology. Series A, Biological sciences and medical sciences 76.12 (2021 Nov 15): 2112-2121.

    Flexibility of equine bioenergetics and muscle plasticity in response to different types of training: An integrative approach, questioning existing paradigms.
    Delesalle CJG
    PloS one 16.4 (2021): e0249922.

    Reading interests of hearing and hearing-impaired children.
    Stoefen-Fisher JM
    American annals of the deaf 130.4 (1985 Oct): 291-6.

    The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.
    Kassel O
    Scientific reports 11.1 (2021 Jun 18): 12904.

    MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides.
    Mayeuf-Louchart A
    Skeletal muscle 13.1 (2023 Aug 23): 14.

    Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes.
    Pang W
    Journal of animal science and biotechnology 15.1 (2024 Jun 2): 73.

    All References

    New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes.
    Rivero JL
    Cell and tissue research 323.2 (2006 Feb): 283-303.

    Sulf1 modulates BMP signaling and is required for somite morphogenesis and development of the horizontal myoseptum.
    Pownall ME
    Developmental biology 378.2 (2013 Jun 15): 107-21.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study.
    Peterson CA
    Journal of translational medicine 14.1 (2016 Sep 29): 284.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Cellular and morphological changes with EAA supplementation before and after total knee arthroplasty.
    Dreyer HC
    Journal of applied physiology (Bethesda, Md. : 1985) 127.2 (2019 Aug 1): 531-545.

    Hypertrophic muscle growth and metabolic efficiency were impaired by chronic heat stress, improved by zilpaterol supplementation, and not affected by ractopamine supplementation in feedlot lambs1.
    Yates DT
    Journal of animal science 97.10 (2019 Oct 3): 4101-4113.

    Skeletal muscle transcriptional networks linked to type I myofiber grouping in Parkinson's disease.
    Bamman MM
    Journal of applied physiology (Bethesda, Md. : 1985) 128.2 (2020 Feb 1): 229-240.

    Countering disuse atrophy in older adults with low-volume leucine supplementation.
    Paddon-Jones D
    Journal of applied physiology (Bethesda, Md. : 1985) 128.4 (2020 Apr 1): 967-977.

    Regular endurance exercise of overloaded muscle of young and old male mice does not attenuate hypertrophy and improves fatigue resistance.
    Degens H
    GeroScience 43.2 (2021 Apr): 741-757.

    Exercise-mediated alteration of hippocampal Dicer mRNA and miRNAs is associated with lower BACE1 gene expression and Aβ(1-42) in female 3xTg-AD mice.
    Peterson CA
    Journal of neurophysiology 124.6 (2020 Dec 1): 1571-1577.

    IGF-1 infusion to fetal sheep increases organ growth but not by stimulating nutrient transfer to the fetus.
    Brown LD
    American journal of physiology. Endocrinology and metabolism 320.3 (2021 Mar 1): E527-E538.

    Mss51 deletion increases endurance and ameliorates histopathology in the mdx mouse model of Duchenne muscular dystrophy.
    Wagner KR
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.2 (2021 Feb): e21276.

    Single-leg exercise training augments in vivo skeletal muscle oxidative flux and vascular content and function in adults with type 2 diabetes.
    Regensteiner JG
    The Journal of physiology 600.4 (2022 Feb): 963-978.

    Home apnea monitoring.
    Hutchins KH
    Pediatric nursing 12.4 (1986 Jul-Aug): 259-62, 304.

    Reduced Physical Activity Alters the Leucine-Stimulated Translatome in Aged Skeletal Muscle.
    Drummond MJ
    The journals of gerontology. Series A, Biological sciences and medical sciences 76.12 (2021 Nov 15): 2112-2121.

    Flexibility of equine bioenergetics and muscle plasticity in response to different types of training: An integrative approach, questioning existing paradigms.
    Delesalle CJG
    PloS one 16.4 (2021): e0249922.

    Reading interests of hearing and hearing-impaired children.
    Stoefen-Fisher JM
    American annals of the deaf 130.4 (1985 Oct): 291-6.

    The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.
    Kassel O
    Scientific reports 11.1 (2021 Jun 18): 12904.

    MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides.
    Mayeuf-Louchart A
    Skeletal muscle 13.1 (2023 Aug 23): 14.

    Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes.
    Pang W
    Journal of animal science and biotechnology 15.1 (2024 Jun 2): 73.

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis.
    Sahenk Z
    Skeletal muscle 7.1 (2017 Dec 14): 27.

    Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.
    McClung JM
    JCI insight 3.21 (2018 Nov 2): .

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging.
    Mann M
    Cell reports 19.11 (2017 Jun 13): 2396-2409.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading.
    Dupont-Versteegden EE
    GeroScience 43.1 (2021 Feb): 65-83.

    Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice.
    Rovere-Querini P
    Aging 12.23 (2020 Nov 26): 23497-23508.

    A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy.
    Zhang ZJ
    Acta neuropathologica communications 9.1 (2021 Feb 8): 23.

    Longitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy.
    Höke A
    Scientific data 6.1 (2019 Sep 24): 179.

    Twenty-one days of low-intensity eccentric training improve morphological characteristics and function of soleus muscles of mdx mice.
    Cornachione AS
    Scientific reports 11.1 (2021 Feb 11): 3579.

    Pioglitazone does not synergize with mirabegron to increase beige fat or further improve glucose metabolism.
    Kern PA
    JCI insight 6.6 (2021 Mar 22): .

    The ROS scavenger PDTC affects adaptation to treadmill running in mice: distinct effects on murine body mass, resting heart rate and skeletal muscle fiber type composition.
    Munz B
    The Journal of experimental biology 224.Pt 6 (2021 Mar 18): .

    Higher strength gain after hypoxic vs normoxic resistance training despite no changes in muscle thickness and fractional protein synthetic rate.
    Deldicque L
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.8 (2021 Aug): e21773.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    β-Cryptoxanthin Improves p62 Accumulation and Muscle Atrophy in the Soleus Muscle of Senescence-Accelerated Mouse-Prone 1 Mice.
    Yamaji R
    Nutrients 12.8 (2020 Jul 22): .

    Myosin heavy chain isoform and metabolic profile differ in beef steaks varying in tenderness.
    Matarneh SK
    Meat science 170. (2020 Dec): 108266.

    A maternal high-fat/low-fiber diet impairs glucose tolerance and induces the formation of glycolytic muscle fibers in neonatal offspring.
    Tan C
    European journal of nutrition 60.5 (2021 Aug): 2709-2718.

    MicroRNA-129-5p inhibits C2C12 myogenesis and represses slow fiber gene expression in vitro.
    Li X
    American journal of physiology. Cell physiology 320.6 (2021 Jun 1): C1031-C1041.

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

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