3.1C12
Catalog Fields
Clone ID/Product Name: 3.1C12
Available to For-Profits: Yes
Alternate Antibody Name: 1C12
Gene Symbol: Cntn2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2229763
Uniprot ID: P22063
Entrez Gene ID: 25356
Clonality: Monoclonal
Immunogen: Rat (E14-E15) spinal cord membranes
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Contactin-2, neuronal marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/15/1992
Antigen Molecular Weight: Predicted: 104 kDa; Apparent: 120 kDa (nonreducing conditions), 135 kDa (reducing conditions)
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University
Antigen Species: rat
Depositor Notes: Stains motoneurons, commissural neurons and central afferents of the DRG. Works on western blots of purified TAG-1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rat
Additional Information: This antibody may work with some mouse strains.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3.1C12 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 3.1C12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
Jessell TM
Neuron 1.2 (1988 Apr): 105-16.