N2.261
Catalog Fields
Clone ID/Product Name: N2.261
Available to For-Profits: Yes
Alternate Antibody Name: Anti-MyHCI/3rd+IIa
Gene Symbol: MYH7
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 4625
Clonality: Monoclonal
Immunogen: partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/2
Epitope Mapped: Yes
Antigen Name: Myosin heavy chain (human all fibers except fast IIB)
Epitope Location or Sequence: N-terminal first 100 aa
Alternate Antigen Name:
Deposit Date: 8/25/1993
Antigen Molecular Weight: 220kDa
Depositor: Blau, H.M.
Antigen Sequence:
Depositor Institution: Stanford University School of Medicine
Antigen Species: Human
Depositor Notes: Fusion: 1983.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rodent, Zebrafish
Additional Information: Stains MyHCIIa and third developmental stage MyHCI, and the epitope on slow MHC is different from that of mAbs A4.951 and A4.951 [PMID 7687223]. Stains all fibers except fast IIb [PMID 3342447; PMID 11005719]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive. RRID: AB_531790
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2.261 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product N2.261)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
Blau HM
Cell 52.4 (1988 Feb 26): 503-13.