A4.74

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SKU: A4.74-s

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Supernatant 1.0 ml

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DSHB Data Sheet

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Clone ID/Product Name: A4.74

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: MYH2

Ab Isotype: MIgG1

Gene Name:

Antibody Registry ID: AB_528383

Uniprot ID: Q9UKX2

RRID:

Entrez Gene ID: 4620

Clonality: Monoclonal

Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle

Clone:

Immunogen Sequence:

Myeloma Strain: 653

Epitope Mapped: No

Antigen Name: Myosin heavy chain (human fast fibers)

Epitope Location or Sequence:

Alternate Antigen Name:

Deposit Date: 8/25/1993

Antigen Molecular Weight: 220kDa

Depositor: Blau, H.M.

Antigen Sequence:

Depositor Institution: Stanford University, Baxter Lab for Stem Cell Biology

Antigen Species: Human

Depositor Notes: Fusion: 1983. Recognizes all fast fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX/D fibers, and doesn't stain IIB.

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Bovine, C. elegans, Horse, Human, Llama, Porcine, Quail, Rodent

Additional Information: stains MyHCIIa [PMID 7687223]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

A4.74 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.74)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

37 References

Initial Publication

IF References

WB References

IHC References

ELISA References

All References

Initial Publication

Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
Blau HM
Cell 52.4 (1988 Feb 26): 503-13.

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A4.74

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