MPIIIB10(1)

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SKU: MPIIIB10(1)

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Supernatant 1.0 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Clone ID/Product Name: MPIIIB10(1)

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: Spp1

Ab Isotype: MIgG1, kappa light chain

Gene Name:

Antibody Registry ID: AB_2286610

Uniprot ID: P08721

RRID:

Entrez Gene ID: 25353

Clonality: Monoclonal

Immunogen: Bone protein fractions

Clone:

Immunogen Sequence: Full length sequence

Myeloma Strain: NS1

Epitope Mapped: No

Antigen Name: Osteopontin

Epitope Location or Sequence:

Alternate Antigen Name:

Deposit Date: 7/11/1989

Antigen Molecular Weight: 34.9 kDa

Depositor: Solursh, M. / Franzen, A.

Antigen Sequence:

Depositor Institution: University of Iowa

Antigen Species: Rat

Depositor Notes: Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin is also known as bone sialoprotein 1, secreted phosphoprotein 1 and early T-lymphocyte activation 1.

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Canine, Human, Mink, Mouse, Rabbit, Rat, Turkey

Additional Information: RRID: AB_2286610

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

MPIIIB10(1) was deposited to the DSHB by Solursh, M. / Franzen, A. (DSHB Hybridoma Product MPIIIB10(1))

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

57 References

Initial Publication

IF References

WB References

IHC References

IP References

FFPE References

FB References

FACS References

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Initial Publication

Bone acidic glycoprotein-75 is a major synthetic product of osteoblastic cells and localized as 75- and/or 50-kDa forms in mineralized phases of bone and growth plate and in serum.
Solursh M
The Journal of biological chemistry 265.25 (1990 Sep 5): 14956-63.

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MPIIIB10(1)

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