GS-9A8
Catalog Fields
Clone ID/Product Name: GS-9A8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: INS
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 3630
Clonality: Monoclonal
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Antigen Name: insulin (proinsulin)
Epitope Location or Sequence: B-C junction of proinsulin (aa 26-37)
Alternate Antigen Name:
Deposit Date: 1/23/2006
Antigen Molecular Weight: Predicted: 9.4 kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: Human
Depositor Notes: This antibody binds to the B-C junction of proinsulin of human and monkey and stains only intact, non-processed proinsulin found in the immature secretory granules of islet beta-cells. It does not cross-react to insulin nor C-peptide. Cross reaction to rodent proinsulins requires aldehyde fixation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Canine, Human, Monkey, Mouse, Rat
Additional Information: Doesn't recognize bovine or porcine proinsulin.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GS-9A8 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GS-9A8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
Steiner DF
Endocrinology 113.6 (1983 Dec): 2135-44.