ADL84.12
Catalog Fields
Product Name/ID: ADL84.12
Available to For-Profits: Yes
Alternate Antibody Name: ADL84; alphaL22-28
Gene Name: Lam
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 33782
Clonality: Monoclonal
Immunogen: Recombinant total protein
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: NS0
Epitope Mapped: Yes
Antigen Name: lamin Dm0
Epitope Location or Sequence: aa 22-28; RPPSAGP
Alternate Antigen Name:
Deposit Date: 3/11/2003
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 kDa
Depositor: Fisher, P. A.
Antigen Sequence:
Depositor Institution: SUNY at Stony Brook, Pharmacological Sciences
Antigen Species: Drosophila
Depositor Notes: Does not react with Sf9 lamin. Reacts only with Dm1 and Dmmit, not Dm2 or Dmmit-s; phosphorylation of S(25) abolishes reactivity; does not react with lamin C.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL84.12 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL84.12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody.
Fisher PA
Journal of cell science 108 ( Pt 9). (1995 Sep): 3137-44.