4F3 anti-discs large

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SKU: 4F3 anti-discs large

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Product Name/ID: 4F3 anti-discs large

Available to For-Profits: Yes

Alternate Antibody Name: discs large 1, isoform L

Gene Name: dlg1

Ab Isotype: MIgG1

Gene Symbol:

Antibody Registry ID: AB_528203

Uniprot ID: P31007

RRID:

Entrez Gene ID: 32083

Clonality: Monoclonal

Immunogen: GST-PDZ2-GST fusion protein; recombinant.

Clone:

Immunogen Sequence: same as the antigen

Myeloma Strain: P3 x 63 Ag8.653

Epitope Mapped: Yes

Antigen Name: Discs large (Drosophila)

Epitope Location or Sequence: a.a 439–756

Alternate Antigen Name:

Deposit Date: 3/14/2002

Antigen Molecular Weight: Predicted: 106.7 kDa

Depositor: Goodman, C.

Antigen Sequence:

Depositor Institution: University of California, Berkeley

Antigen Species: Drosophila

Depositor Notes:

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Drosophila

Additional Information: RRID: AB_528203

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

4F3 anti-discs large was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4F3 anti-discs large)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

68 References

Initial Publication

IF References

WB References

IHC References

IP References

All References

Initial Publication

Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix.
Goodman CS
Neuron 32.3 (2001 Nov 8): 415-24.

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4F3 anti-discs large

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