anti-vasa
Catalog Fields
Product Name/ID: anti-vasa
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: vas
Ab Isotype: RIgM, kappa light chain
Gene Symbol:
Entrez Gene ID: 26067080
Clonality: Monoclonal
Immunogen: Recombinant fusion protein GST-vasa
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain:
Epitope Mapped: No
Antigen Name: vasa
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/9/2007
Antigen Molecular Weight: Predicted: 72 kDa; Apparent 72 kDa
Depositor: Spradling, A.C. / Williams, D.
Antigen Sequence:
Depositor Institution: Carnegie Institute of Washington
Antigen Species: Drosophila
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Vasa is a germline specific marker
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-vasa was deposited to the DSHB by Spradling, A.C. / Williams, D. (DSHB Hybridoma Product anti-vasa)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
20 References
Initial Publication
Reduced fertility of Drosophila melanogaster hybrid male rescue (Hmr) mutant females is partially complemented by Hmr orthologs from sibling species.
Barbash DA
Genetics 181.4 (2009 Apr): 1437-50.