7G10 anti-Fasciclin III
Catalog Fields
Product Name/ID: 7G10 anti-Fasciclin III
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Fas3
Ab Isotype: MIgG2a
Gene Symbol:
Entrez Gene ID: 35097
Clonality: Monoclonal
Immunogen: Membrane preparation from nerve cord and cultures of primary neurons (10-13 hr development).
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Fasciclin III (Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight: Multiple isoforms; Apparent: 46, 59, 66, and 80 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: Fas III is expressed on a subset of neurons and axons in the CNS, patches of ectoderm, ectodermal stripes, parts of the hindgut and foregut, visceral mesoderm, and the salivary gland. The antibody can be used to stain dissections without detergent as the epitope is on the extracellular domain of the protein. After 10 hrs in development, ectodermal staining will obscure CNS in whole mount preps and therefore dissected preparations are recommended for embryos staining past this age. During larval development, staining is present on a subset of neurons, the epithelial layer of the discs and a cyclone fence pattern in the eye disc. It is especially useful for following the development of the RP1 and RP3 neurons. The antibody works for affinity purification but does not work for immunoprecipitation or western.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This antibody may work for Immunoprecipitation. While the Depositor clearly stated that it doesn't work for immunoprecipitation (see Depositor Notes), the Initial Publication, in which the Depositor is the last author, reports the succesful use of 7G10 for the immunoprecipitation of Fasciclin III.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7G10 anti-Fasciclin III was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 7G10 anti-Fasciclin III)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
36 References
Initial Publication
Characterization and cloning of fasciclin III: a glycoprotein expressed on a subset of neurons and axon pathways in Drosophila.
Goodman CS
Cell 48.6 (1987 Mar 27): 975-88.