5F10
Catalog Fields
Product Name/ID: 5F10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Clonality: Monoclonal
Immunogen: synthetic AST7 (APSGAQRLYGFGL), N-terminal coupled to BSA
Clone:
Immunogen Sequence: peptide
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: allatostatin (Ast7)
Epitope Location or Sequence: likely YGFGL
Alternate Antigen Name:
Deposit Date: 7/10/2003
Antigen Molecular Weight: Predicted: 39 kDa (propeptide), 1.3 kDa (ATS7)
Depositor: Stay, B.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Cockroach
Depositor Notes: Works on many insect species and many invertebrates. Some cross reaction with leu-callatostatin 1. Stains neural tissue, gut endocrine cells and ovary.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Cockroach, Copepod, Crab, Drosophila melanogaster, Earthworm, Insect, Invertebrate, Schmidtea mediterranea, Snail
Additional Information: Stains type A allatostatins.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5F10 was deposited to the DSHB by Stay, B. (DSHB Hybridoma Product 5F10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata.
Woodhead AP
Cell and tissue research 270.1 (1992 Oct): 15-23.