ChAT4B1
Catalog Fields
Product Name/ID: ChAT4B1
Available to For-Profits: Yes
Alternate Antibody Name: 4B1
Gene Name: cha
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 42249
Clonality: Monoclonal
Immunogen: Recombinant fusion protein
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: choline acetyltransferase
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/6/2002
Antigen Molecular Weight: 81 kDa; cleaved into three chains of 13, 54, and 67 kDa
Depositor: Salvaterra, P.M.
Antigen Sequence:
Depositor Institution: City of Hope, Beckman Research Institute
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila, Manduca sexta
Additional Information: Choline O-acetyltransferase catalyzes the reversible synthesis of acetylcholine from acetyl CoA and choline at cholinergic synapses.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ChAT4B1 was deposited to the DSHB by Salvaterra, P.M. (DSHB Hybridoma Product ChAT4B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
23 References
Initial Publication
Analysis of choline acetyltransferase protein in temperature sensitive mutant flies using newly generated monoclonal antibody.
Salvaterra P
Neuroscience research 24.3 (1996 Feb): 237-43.