PDF C7
Catalog Fields
Product Name/ID: PDF C7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Pdf
Ab Isotype: MIgG2b, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_760350 AB_2315084
Uniprot ID: O96690
Entrez Gene ID: 43193
Clonality: Monoclonal
Immunogen: amidated pigment dispersing factor neuropeptide
Clone:
Immunogen Sequence: NSELINSLLSLPKNMNDA-NH2
Myeloma Strain: SP2-0/Ag14
Epitope Mapped: Yes
Antigen Name: Pigment-dispersing factor neuropeptide
Epitope Location or Sequence: NSELINSLLSLPKNMNDA-NH2
Alternate Antigen Name:
Deposit Date: 11/10/2006
Antigen Molecular Weight: Predicted: PDF, 2kDa; PDF precursor, 11.5kDa
Depositor: Blau, Justin
Antigen Sequence:
Depositor Institution: New York University, Biology
Antigen Species: Drosophila
Depositor Notes: Fusion date: 2005. Recognizes Drosophila larval and adult brains.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Cabbage root fly, Cockroach, Drosophila, Mosquito
Additional Information: The neuropeptide PDF represents the C-terminus portion (aa 83-100) of the Protein PDF [Uniprot O96690]. The neuropeptide PDF regulates circadian loco motor rhythms.
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PDF C7 was deposited to the DSHB by Blau, Justin (DSHB Hybridoma Product PDF C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
40 References
Initial Publication
The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period.
Blau J
The Journal of neuroscience : the official journal of the Society for Neuroscience 25.22 (2005 Jun 1): 5430-7.