znt-1

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SKU: znt-1-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: znt-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_531911 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Adult brain membranes. Boosts: adult CNS membranes
Clone:
Immunogen Sequence:
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antigen Name: notochord and neuropil marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/30/1996
Antigen Molecular Weight:
Depositor: Trevarrow, B.
Antigen Sequence:
Depositor Institution: University of Oregon, Institute of Neuroscience
Antigen Species: zebrafish
Depositor Notes: Doesn't work on Xenopus, chicken, shark, medaka or trout. Works on zebrafish embryos/larva and adults, betta fish, Haplochromis burtoni and tunicates. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat) the cell surface of notochord, actinotrichia, neuropil, pectoral fin tip and otocyst.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bony fish, Tunicate, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
znt-1 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product znt-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis.
    Cheng SH
    Toxicological sciences : an official journal of the Society of Toxicology 73.1 (2003 May): 149-59.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis.
    Cheng SH
    Toxicological sciences : an official journal of the Society of Toxicology 73.1 (2003 May): 149-59.

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