anti-Bicaudal-D 4C2
Catalog Fields
Clone ID/Product Name: anti-Bicaudal-D 4C2
Available to For-Profits: Yes
Alternate Antibody Name: 4C2
Gene Symbol: BicD
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 35051
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Bicaudal-D
Epitope Location or Sequence: N-terminal (aa 1-543)
Alternate Antigen Name:
Deposit Date: 3/20/2003
Antigen Molecular Weight: Predicted: 89kDa; Apparent: 97kDa
Depositor: Steward, R.
Antigen Sequence:
Depositor Institution: Rutgers University, Waksman Institute
Antigen Species: Drosophila
Depositor Notes: Fail to detect BicD in some loss-of-function bicD alleles.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: 4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Bicaudal-D 4C2 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-Bicaudal-D 4C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
22 References
Initial Publication
Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
Steward R
Cell 67.5 (1991 Nov 29): 917-26.