QCPN

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SKU: QCPN

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: QCPN
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_531886 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Quail wing bud ZPA; HH Stage 21-24
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63 - Ag 8.653
Epitope Mapped: No
Antigen Name: Quail cell marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/6/1992
Antigen Molecular Weight:
Depositor: Carlson, B.M. / Carlson, J.A.
Antigen Sequence:
Depositor Institution: University of Michigan
Antigen Species: Quail
Depositor Notes: This antibody is used to detect quail cells in chick-quail chimera xenographs.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Quail
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
QCPN was deposited to the DSHB by Carlson, B.M. / Carlson, J.A. (DSHB Hybridoma Product QCPN)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

34 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Origins of the avian neural crest: the role of neural plate-epidermal interactions.
    Bronner-Fraser M
    Development (Cambridge, England) 121.2 (1995 Feb): 525-38.

    IF References
    WB References
    IHC References

    Intrinsic properties guide proximal abducens and oculomotor nerve outgrowth in avian embryos.
    Sours E
    Developmental neurobiology 72.2 (2012 Feb): 167-85.

    Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
    Oláh I
    Development (Cambridge, England) 137.18 (2010 Sep): 3019-23.

    Mesenchyme-dependent BMP signaling directs the timing of mandibular osteogenesis.
    Schneider RA
    Development (Cambridge, England) 135.7 (2008 Apr): 1223-34.

    Foregut endoderm is specified early in avian development through signal(s) emanating from Hensen's node or its derivatives.
    Yasugi S
    Mechanisms of development 125.5-6 (2008 May-Jun): 377-95.

    Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 236.1 (2007 Jan): 73-83.

    Intestinal coelomic transplants: a novel method for studying enteric nervous system development.
    Goldstein AM
    Cell and tissue research 326.1 (2006 Oct): 43-55.

    Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system.
    Goldstein AM
    Developmental biology 293.1 (2006 May 1): 203-17.

    Quail-duck chimeras reveal spatiotemporal plasticity in molecular and histogenic programs of cranial feather development.
    Schneider RA
    Development (Cambridge, England) 132.7 (2005 Apr): 1499-509.

    Neurally mediated renal vasoconstriction during isometric muscle contraction in cats.
    Mitchell JH
    The American journal of physiology 262.3 Pt 2 (1992 Mar): H833-8.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Extracellular matrix of different composition supports the various splenic compartments of guinea fowl ( Numida meleagris).
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Microvascular assembly and cell invasion in chick mesonephros grafted onto chorioallantoic membrane.
    Ruberte J
    Journal of anatomy 202.2 (2003 Feb): 213-25.

    The cellular and molecular origins of beak morphology.
    Helms JA
    Science (New York, N.Y.) 299.5606 (2003 Jan 24): 565-8.

    Pre-gut endoderm of chick embryos is regionalized by 1.5 days of development.
    Yasugi S
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 33-47.

    Competence, specification and commitment in otic placode induction.
    Bronner-Fraser M
    Development (Cambridge, England) 127.16 (2000 Aug): 3489-99.

    Competence, specification and induction of Pax-3 in the trigeminal placode.
    Bronner-Fraser M
    Development (Cambridge, England) 126.1 (1999 Jan): 147-56.

    Origins of the avian neural crest: the role of neural plate-epidermal interactions.
    Bronner-Fraser M
    Development (Cambridge, England) 121.2 (1995 Feb): 525-38.

    Multiple origins, migratory paths and molecular profiles of cells populating the avian interpeduncular nucleus.
    Aroca P
    Developmental biology 361.1 (2012 Jan 1): 12-26.

    Retinoic acid upregulates ret and induces chain migration and population expansion in vagal neural crest cells to colonise the embryonic gut.
    Newgreen DF
    PloS one 8.5 (2013): e64077.

    The amniote paratympanic organ develops from a previously undiscovered sensory placode.
    Baker CV
    Nature communications 3. (2012): 1041.

    Origin and plasticity of the subdivisions of the inferior olivary complex.
    Bloch-Gallego E
    Developmental biology 371.2 (2012 Nov 15): 215-26.

    Fate map of the chicken otic placode.
    Hidalgo-Sánchez M
    Development (Cambridge, England) 141.11 (2014 Jun): 2302-12.

    Resident progenitors, not exogenous migratory cells, generate the majority of visceral mesothelium in organogenesis.
    Bader DM
    Developmental biology 391.2 (2014 Jul 15): 125-32.

    The neural crest, a multifaceted structure of the vertebrates.
    Le Douarin NM
    Birth defects research. Part C, Embryo today : reviews 102.3 (2014 Sep): 187-209.

    The unique axon trajectory of the accessory nerve is determined by intrinsic properties of the neural tube in the avian embryo.
    Huang R
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 205. (2016 May): 85-9.

    Environmental and intrinsic modulations of venous differentiation.
    Pardanaud L
    Cellular and molecular life sciences : CMLS 79.9 (2022 Aug 20): 491.

    FFPE References
    All References

    Intrinsic properties guide proximal abducens and oculomotor nerve outgrowth in avian embryos.
    Sours E
    Developmental neurobiology 72.2 (2012 Feb): 167-85.

    Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
    Oláh I
    Development (Cambridge, England) 137.18 (2010 Sep): 3019-23.

    Mesenchyme-dependent BMP signaling directs the timing of mandibular osteogenesis.
    Schneider RA
    Development (Cambridge, England) 135.7 (2008 Apr): 1223-34.

    Foregut endoderm is specified early in avian development through signal(s) emanating from Hensen's node or its derivatives.
    Yasugi S
    Mechanisms of development 125.5-6 (2008 May-Jun): 377-95.

    Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 236.1 (2007 Jan): 73-83.

    Intestinal coelomic transplants: a novel method for studying enteric nervous system development.
    Goldstein AM
    Cell and tissue research 326.1 (2006 Oct): 43-55.

    Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system.
    Goldstein AM
    Developmental biology 293.1 (2006 May 1): 203-17.

    Quail-duck chimeras reveal spatiotemporal plasticity in molecular and histogenic programs of cranial feather development.
    Schneider RA
    Development (Cambridge, England) 132.7 (2005 Apr): 1499-509.

    Neurally mediated renal vasoconstriction during isometric muscle contraction in cats.
    Mitchell JH
    The American journal of physiology 262.3 Pt 2 (1992 Mar): H833-8.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Extracellular matrix of different composition supports the various splenic compartments of guinea fowl ( Numida meleagris).
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Microvascular assembly and cell invasion in chick mesonephros grafted onto chorioallantoic membrane.
    Ruberte J
    Journal of anatomy 202.2 (2003 Feb): 213-25.

    The cellular and molecular origins of beak morphology.
    Helms JA
    Science (New York, N.Y.) 299.5606 (2003 Jan 24): 565-8.

    Pre-gut endoderm of chick embryos is regionalized by 1.5 days of development.
    Yasugi S
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 33-47.

    Competence, specification and commitment in otic placode induction.
    Bronner-Fraser M
    Development (Cambridge, England) 127.16 (2000 Aug): 3489-99.

    Competence, specification and induction of Pax-3 in the trigeminal placode.
    Bronner-Fraser M
    Development (Cambridge, England) 126.1 (1999 Jan): 147-56.

    Origins of the avian neural crest: the role of neural plate-epidermal interactions.
    Bronner-Fraser M
    Development (Cambridge, England) 121.2 (1995 Feb): 525-38.

    Multiple origins, migratory paths and molecular profiles of cells populating the avian interpeduncular nucleus.
    Aroca P
    Developmental biology 361.1 (2012 Jan 1): 12-26.

    Retinoic acid upregulates ret and induces chain migration and population expansion in vagal neural crest cells to colonise the embryonic gut.
    Newgreen DF
    PloS one 8.5 (2013): e64077.

    The amniote paratympanic organ develops from a previously undiscovered sensory placode.
    Baker CV
    Nature communications 3. (2012): 1041.

    Origin and plasticity of the subdivisions of the inferior olivary complex.
    Bloch-Gallego E
    Developmental biology 371.2 (2012 Nov 15): 215-26.

    Fate map of the chicken otic placode.
    Hidalgo-Sánchez M
    Development (Cambridge, England) 141.11 (2014 Jun): 2302-12.

    Resident progenitors, not exogenous migratory cells, generate the majority of visceral mesothelium in organogenesis.
    Bader DM
    Developmental biology 391.2 (2014 Jul 15): 125-32.

    The neural crest, a multifaceted structure of the vertebrates.
    Le Douarin NM
    Birth defects research. Part C, Embryo today : reviews 102.3 (2014 Sep): 187-209.

    The unique axon trajectory of the accessory nerve is determined by intrinsic properties of the neural tube in the avian embryo.
    Huang R
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 205. (2016 May): 85-9.

    Environmental and intrinsic modulations of venous differentiation.
    Pardanaud L
    Cellular and molecular life sciences : CMLS 79.9 (2022 Aug 20): 491.

    Foregut Mesenchyme Contributes Cells to Islets during Pancreatic Development in a 3-Dimensional Avian Model.
    Johnson PR
    Organogenesis 1.2 (2004 Oct): 45-51.

    Does the subepicardial mesenchyme contribute myocardioblasts to the myocardium of the chick embryo heart? A quail-chick chimera study tracing the fate of the epicardial primordium.
    Männer J
    The Anatomical record 255.2 (1999 Jun 1): 212-26.

    Neural crest can form cartilages normally derived from mesoderm during development of the avian head skeleton.
    Schneider RA
    Developmental biology 208.2 (1999 Apr 15): 441-55.

    Rhombencephalic neural crest segmentation is preserved throughout craniofacial ontogeny.
    Lumsden A
    Development (Cambridge, England) 122.10 (1996 Oct): 3229-42.

    A bird's eye view of enteric nervous system development: lessons from the avian embryo.
    Nagy N
    Pediatric research 64.4 (2008 Oct): 326-33.

    Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm.
    Jessell TM
    Cell 82.6 (1995 Sep 22): 969-79.

    Development of chicken aortic smooth muscle: expression of cytoskeletal and basement membrane proteins defines two distinct cell phenotypes emerging from a common lineage.
    Christ B
    Cellular & molecular biology research 41.4 (1995): 241-9.

    A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras.
    Ohki-Hamazaki H
    Nature communications 4. (2013): 1372.

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