M1-B4
Catalog Fields
Clone ID/Product Name: M1-B4
Available to For-Profits: Yes
Alternate Antibody Name: tenascin
Gene Symbol: TNC
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 396440
Clonality: Monoclonal
Immunogen: Pepsin digest of type V collagen
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Tenascin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/8/1986
Antigen Molecular Weight: 150-240 kDa
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins University School of Medicine
Antigen Species: Chick
Depositor Notes: The M1-B4 epitope is preserved is Zenker's fixative or freeze-substituted paraffin sections (see PMID: 2464073). Historically, this antigen has been known as myotendinous antigen, neuronectin, glioma-associated ECM antigen and cytotactin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Newt
Additional Information: RRID: AB_528488
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M1-B4 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product M1-B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
Fambrough DM
The Journal of cell biology 98.6 (1984 Jun): 1926-36.