Cq4
Catalog Fields
Clone ID/Product Name: Cq4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: crb
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 42896
Clonality: Monoclonal
Immunogen: Recombinant fusion protein between beta-galactosidase and aa 737-1703 of Drosophila Crumbs protein.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: Crumbs Drosophila protein (extracellular domain)
Epitope Location or Sequence: aa 737-1703
Alternate Antigen Name:
Deposit Date: 9/27/2000
Antigen Molecular Weight: Predicted: 234 kDa
Depositor: Knust, E.
Antigen Sequence:
Depositor Institution: Max Planck Institute of Molecular Cell Biology and Genetics
Antigen Species: Drosophila
Depositor Notes: crossreacts with Drosophila virilis, not with mouse or C. elegans. Crumbs protein is expressed in all epithelia of ectodermal origin in the Drosophila embryo from gastrulation onwards and in all imaginal discs and the follicle epithelium. It is restricted to a region apical to the zonula adherens. Good apical marker for epithelial cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila melanogaster, Drosophila virilis
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cq4 was deposited to the DSHB by Knust, E. (DSHB Hybridoma Product Cq4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Crumbs and stardust act in a genetic pathway that controls the organization of epithelia in Drosophila melanogaster.
Knust E
Developmental biology 159.1 (1993 Sep): 311-26.