CF.6G11

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SKU: CF.6G11

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: CF.6G11
Available to For-Profits: Yes
Alternate Antibody Name: PS3, betaPS
Gene Symbol: mys
Ab Isotype: MIgG2b, kappa light chain
Gene Name:
Antibody Registry ID: AB_528310 
Uniprot ID: P11584 
RRID:  
Entrez Gene ID: 44885 
Clonality: Monoclonal
Immunogen: Sonicated imaginal discs
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: P3/NSI
Epitope Mapped: No
Antigen Name: integrin betaPS (myospheroid)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2002
Antigen Molecular Weight: 93 kDa
Depositor: Brower, D.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Drosophila
Depositor Notes: This antibody doesn't block function.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. Beta-PS associates with either alpha-PS1, alpha-PS2, alpha-PS3, alpha-PS4 or alpha-PS5.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CF.6G11 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product CF.6G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

36 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    IF References

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.
    Yang X
    Developmental biology 325.1 (2009 Jan 1): 15-23.

    The cadherin Fat2 is required for planar cell polarity in the Drosophila ovary.
    Dahmann C
    Development (Cambridge, England) 136.24 (2009 Dec): 4123-32.

    The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis.
    Hsu T
    Molecular and cellular biology 29.17 (2009 Sep): 4679-90.

    Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
    Stenmark H
    PloS one 4.2 (2009): e4354.

    Membrane protein location-dependent regulation by PI3K (III) and rabenosyn-5 in Drosophila wing cells.
    Goto S
    PloS one 4.10 (2009 Oct 2): e7306.

    The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium.
    Brown NH
    Journal of cell science 122.Pt 23 (2009 Dec 1): 4363-74.

    The proprioceptive and contractile systems in Drosophila are both patterned by the EGR family transcription factor Stripe.
    Salzberg A
    Developmental biology 337.2 (2010 Jan 15): 458-70.

    The Rap1 guanine nucleotide exchange factor C3G is required for preservation of larval muscle integrity in Drosophila melanogaster.
    Palmer RH
    PloS one 5.3 (2010 Mar 3): e9403.

    Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome.
    Nishihara S
    PloS one 5.7 (2010 Jul 13): e11557.

    Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila.
    Pret AM
    PloS one 5.11 (2010 Nov 11): e13946.

    Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression.
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    The Putzig-NURF nucleosome remodeling complex is required for ecdysone receptor signaling and innate immunity in Drosophila melanogaster.
    Nagel AC
    Genetics 188.1 (2011 May): 127-39.

    The Rho-family GTPase Rac1 regulates integrin localization in Drosophila immunosurveillance cells.
    Williams MJ
    PloS one 6.5 (2011): e19504.

    A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted.
    Kadrmas JL
    Journal of cell science 125.Pt 13 (2012 Jul 1): 3185-94.

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    Loss of a Clueless-dGRASP complex results in ER stress and blocks Integrin exit from the perinuclear endoplasmic reticulum in Drosophila larval muscle.
    Geisbrecht ER
    Biology open 4.5 (2015 Apr 10): 636-48.

    Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila.
    Horne-Badovinac S
    The Journal of cell biology 200.6 (2013 Mar 18): 721-9.

    Focal adhesion kinase is not required for integrin function or viability in Drosophila.
    Palmer RH
    Development (Cambridge, England) 131.23 (2004 Dec): 5795-805.

    Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary.
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Developmental analysis of Drosophila position-specific antigens.
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    Thrombospondin expression in myofibers stabilizes muscle membranes.
    Molkentin JD
    eLife 5. (2016 Sep 26): .

    Dendritic space-filling requires a neuronal type-specific extracellular permissive signal in Drosophila.
    Han C
    Proceedings of the National Academy of Sciences of the United States of America 114.38 (2017 Sep 19): E8062-E8071.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.
    Dahmann C
    Nature communications 9.1 (2018 Nov 5): 4620.

    Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis.
    Li Z
    Scientific reports 9.1 (2019 Apr 16): 6118.

    Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix.
    Mlodzik M
    Open biology 9.8 (2019 Aug 30): 190148.

    Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
    Tanentzapf G
    Current biology : CB 30.17 (2020 Sep 7): 3316-3329.e5.

    Suppression of Patronin deficiency by altered Hippo signaling in Drosophila organ development.
    Choi KW
    Cell death and differentiation 28.1 (2021 Jan): 233-250.

    Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes.
    Mishra RK
    Scientific reports 11.1 (2021 Jan 19): 1838.

    WB References
    IHC References

    Genetic analysis on the role of integrin during axon guidance in Drosophila.
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    IP References

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    FACS References

    Differences in regulation of Drosophila and vertebrate integrin affinity by talin.
    Shattil SJ
    Molecular biology of the cell 19.8 (2008 Aug): 3589-98.

    All References

    Genetic analysis on the role of integrin during axon guidance in Drosophila.
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.
    Yang X
    Developmental biology 325.1 (2009 Jan 1): 15-23.

    The cadherin Fat2 is required for planar cell polarity in the Drosophila ovary.
    Dahmann C
    Development (Cambridge, England) 136.24 (2009 Dec): 4123-32.

    The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis.
    Hsu T
    Molecular and cellular biology 29.17 (2009 Sep): 4679-90.

    Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
    Stenmark H
    PloS one 4.2 (2009): e4354.

    Membrane protein location-dependent regulation by PI3K (III) and rabenosyn-5 in Drosophila wing cells.
    Goto S
    PloS one 4.10 (2009 Oct 2): e7306.

    The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium.
    Brown NH
    Journal of cell science 122.Pt 23 (2009 Dec 1): 4363-74.

    The proprioceptive and contractile systems in Drosophila are both patterned by the EGR family transcription factor Stripe.
    Salzberg A
    Developmental biology 337.2 (2010 Jan 15): 458-70.

    The Rap1 guanine nucleotide exchange factor C3G is required for preservation of larval muscle integrity in Drosophila melanogaster.
    Palmer RH
    PloS one 5.3 (2010 Mar 3): e9403.

    Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome.
    Nishihara S
    PloS one 5.7 (2010 Jul 13): e11557.

    Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila.
    Pret AM
    PloS one 5.11 (2010 Nov 11): e13946.

    Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression.
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    The Putzig-NURF nucleosome remodeling complex is required for ecdysone receptor signaling and innate immunity in Drosophila melanogaster.
    Nagel AC
    Genetics 188.1 (2011 May): 127-39.

    The Rho-family GTPase Rac1 regulates integrin localization in Drosophila immunosurveillance cells.
    Williams MJ
    PloS one 6.5 (2011): e19504.

    A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted.
    Kadrmas JL
    Journal of cell science 125.Pt 13 (2012 Jul 1): 3185-94.

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    Loss of a Clueless-dGRASP complex results in ER stress and blocks Integrin exit from the perinuclear endoplasmic reticulum in Drosophila larval muscle.
    Geisbrecht ER
    Biology open 4.5 (2015 Apr 10): 636-48.

    Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila.
    Horne-Badovinac S
    The Journal of cell biology 200.6 (2013 Mar 18): 721-9.

    Focal adhesion kinase is not required for integrin function or viability in Drosophila.
    Palmer RH
    Development (Cambridge, England) 131.23 (2004 Dec): 5795-805.

    Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary.
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Developmental analysis of Drosophila position-specific antigens.
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    Thrombospondin expression in myofibers stabilizes muscle membranes.
    Molkentin JD
    eLife 5. (2016 Sep 26): .

    Dendritic space-filling requires a neuronal type-specific extracellular permissive signal in Drosophila.
    Han C
    Proceedings of the National Academy of Sciences of the United States of America 114.38 (2017 Sep 19): E8062-E8071.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.
    Dahmann C
    Nature communications 9.1 (2018 Nov 5): 4620.

    Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis.
    Li Z
    Scientific reports 9.1 (2019 Apr 16): 6118.

    Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix.
    Mlodzik M
    Open biology 9.8 (2019 Aug 30): 190148.

    Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
    Tanentzapf G
    Current biology : CB 30.17 (2020 Sep 7): 3316-3329.e5.

    Suppression of Patronin deficiency by altered Hippo signaling in Drosophila organ development.
    Choi KW
    Cell death and differentiation 28.1 (2021 Jan): 233-250.

    Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes.
    Mishra RK
    Scientific reports 11.1 (2021 Jan 19): 1838.

    Differences in regulation of Drosophila and vertebrate integrin affinity by talin.
    Shattil SJ
    Molecular biology of the cell 19.8 (2008 Aug): 3589-98.

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