BDNF-#9
Catalog Fields
Clone ID/Product Name: BDNF-#9
Available to For-Profits: Yes
Alternate Antibody Name: mAb#9
Gene Symbol: BDNF
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2617199
Uniprot ID: P23560
Entrez Gene ID: 627
Clonality: Monoclonal
Immunogen: Fish and Human BDNF
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63.Ag 8.635
Epitope Mapped: No
Antigen Name: Mature brain-derived neurotrophic factor
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/2/2012
Antigen Molecular Weight: Apparent: 14 kDa
Depositor: Barde, Y.-A.
Antigen Sequence:
Depositor Institution: University of Basel, Biozentrum
Antigen Species: Fish/Human
Depositor Notes: BDNF-#9 blocks the biological activity of Xyphophorus and mammalian BDNF. For immunostaining: perfusion fixation with 4% paraformaldehyde, 0.1% glutaraldehyde, 0.3% picric acid.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Fish, Human, Mammal, Mouse, Rat
Additional Information: BDNF-#9 does not recognize BDNF on western blots but mAb BDNF-#1 (a different clone from the same fusion; available in the DSHB collection) reacts specifically with BDNF by western blot. BDNF-#9 doesn't appear to recognize chick BDNF. This antibody also recognizes pro-BDNF. For ELISA, BDNF-#9 is often used in combination with BDNF-#1 that recognizes a different epitope on BDNF.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BDNF-#9 was deposited to the DSHB by Barde, Y.-A. (DSHB Hybridoma Product BDNF-#9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Brain-derived neurotrophic factor levels in the nervous system of wild-type and neurotrophin gene mutant mice.
Barde YA
Journal of neurochemistry 72.5 (1999 May): 1930-8.