7G1-1

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SKU: 7G1-1-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 7G1-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Fmr1
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_528251 
Uniprot ID: P35922 
RRID:  
Entrez Gene ID: 14265 
Clonality: Monoclonal
Immunogen: Fmrp-6X-his fusion protein.
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: FMR1
Epitope Location or Sequence: a.a. 354-368 [seq: KHLDTKENTHFSQPN}
Alternate Antigen Name:
Deposit Date: 3/7/2003
Antigen Molecular Weight: ~70-80kDa
Depositor: Warren, S.T.
Antigen Sequence:
Depositor Institution: Emory University
Antigen Species: Mouse
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7G1-1 was deposited to the DSHB by Warren, S.T. (DSHB Hybridoma Product 7G1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

23 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Myosin Va is required for the transport of fragile X mental retardation protein (FMRP) granules.
    McCaffrey MW
    Biology of the cell 106.2 (2014 Feb): 57-71.

    Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
    Boccaccio GL
    The Journal of cell biology 195.7 (2011 Dec 26): 1141-57.

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    WB References

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124.
    Gao FB
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.39 (2011 Sep 28): 13705-9.

    FMRP regulates the transition from radial glial cells to intermediate progenitor cells during neocortical development.
    Xie Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.4 (2011 Jan 26): 1427-39.

    Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse.
    Hampson DR
    Molecular and cellular neurosciences 46.3 (2011 Mar): 563-72.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    The RNA binding and transport proteins staufen and fragile X mental retardation protein are expressed by rat primary afferent neurons and localize to peripheral and central axons.
    Hargreaves KM
    Neuroscience 141.4 (2006 Sep 15): 2107-16.

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Small noncoding vault RNA modulates synapse formation by amplifying MAPK signaling.
    Araki T
    The Journal of cell biology 220.2 (2021 Feb 1): .

    IHC References

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    IP References

    Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.
    Warren ST
    Cell 107.4 (2001 Nov 16): 477-87.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density.
    Dolphin AC
    Nature communications 5. (2014 Apr 7): 3628.

    Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures.
    Benke TA
    Neurobiology of disease 59. (2013 Nov): 1-17.

    Fragile X mental retardation protein is required for rapid experience-dependent regulation of the potassium channel Kv3.1b.
    Kaczmarek LK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.31 (2010 Aug 4): 10263-71.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism.
    Darnell RB
    Cell 146.2 (2011 Jul 22): 247-61.

    Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus.
    Wong RK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.2 (2011 Jan 12): 725-34.

    An alternative mode of microRNA target recognition.
    Darnell RB
    Nature structural & molecular biology 19.3 (2012 Feb 12): 321-7.

    Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
    Bassell GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10624-38.

    FMRP mediates mGluR5-dependent translation of amyloid precursor protein.
    Malter JS
    PLoS biology 5.3 (2007 Mar): e52.

    Reply to Bagni: On BC1 RNA and the fragile X mental retardation protein.
    Tiedge H
    Proceedings of the National Academy of Sciences of the United States of America 105.22 (2008 Jun 3): E29.

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain.
    Smalheiser NR
    Journal of neurochemistry 106.2 (2008 Jul): 650-61.

    Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
    Dziembowska M
    Molecular neurobiology 56.4 (2019 Apr): 2741-2759.

    Epitope Map References
    Gel Supershift References

    Post-transcriptional regulation of the let-7 microRNA during neural cell specification.
    Nitsch R
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21.2 (2007 Feb): 415-26.

    All References

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    Myosin Va is required for the transport of fragile X mental retardation protein (FMRP) granules.
    McCaffrey MW
    Biology of the cell 106.2 (2014 Feb): 57-71.

    Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
    Boccaccio GL
    The Journal of cell biology 195.7 (2011 Dec 26): 1141-57.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124.
    Gao FB
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.39 (2011 Sep 28): 13705-9.

    FMRP regulates the transition from radial glial cells to intermediate progenitor cells during neocortical development.
    Xie Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.4 (2011 Jan 26): 1427-39.

    Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse.
    Hampson DR
    Molecular and cellular neurosciences 46.3 (2011 Mar): 563-72.

    The RNA binding and transport proteins staufen and fragile X mental retardation protein are expressed by rat primary afferent neurons and localize to peripheral and central axons.
    Hargreaves KM
    Neuroscience 141.4 (2006 Sep 15): 2107-16.

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Small noncoding vault RNA modulates synapse formation by amplifying MAPK signaling.
    Araki T
    The Journal of cell biology 220.2 (2021 Feb 1): .

    Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.
    Warren ST
    Cell 107.4 (2001 Nov 16): 477-87.

    Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density.
    Dolphin AC
    Nature communications 5. (2014 Apr 7): 3628.

    Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures.
    Benke TA
    Neurobiology of disease 59. (2013 Nov): 1-17.

    Fragile X mental retardation protein is required for rapid experience-dependent regulation of the potassium channel Kv3.1b.
    Kaczmarek LK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.31 (2010 Aug 4): 10263-71.

    FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism.
    Darnell RB
    Cell 146.2 (2011 Jul 22): 247-61.

    Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus.
    Wong RK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.2 (2011 Jan 12): 725-34.

    An alternative mode of microRNA target recognition.
    Darnell RB
    Nature structural & molecular biology 19.3 (2012 Feb 12): 321-7.

    Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
    Bassell GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10624-38.

    FMRP mediates mGluR5-dependent translation of amyloid precursor protein.
    Malter JS
    PLoS biology 5.3 (2007 Mar): e52.

    Reply to Bagni: On BC1 RNA and the fragile X mental retardation protein.
    Tiedge H
    Proceedings of the National Academy of Sciences of the United States of America 105.22 (2008 Jun 3): E29.

    Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain.
    Smalheiser NR
    Journal of neurochemistry 106.2 (2008 Jul): 650-61.

    Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
    Dziembowska M
    Molecular neurobiology 56.4 (2019 Apr): 2741-2759.

    Post-transcriptional regulation of the let-7 microRNA during neural cell specification.
    Nitsch R
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21.2 (2007 Feb): 415-26.

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