4C9H4 anti-peanut
Catalog Fields
Clone ID/Product Name: 4C9H4 anti-peanut
Available to For-Profits: Yes
Alternate Antibody Name: 4C9
Gene Symbol: pnut
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 35801
Clonality: Monoclonal
Immunogen: GST-fusion protein containing the amino-terminal 116 aa of the peanut protein.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Antigen Name: peanut gene protein product
Epitope Location or Sequence: amino-terminal 116 aa
Alternate Antigen Name:
Deposit Date: 8/31/1994
Antigen Molecular Weight: Predicted: 60 kDa; Apparent: 60 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: 4C9H4 is an excellent marker for cytokinesis, and labels the cleavage furrow of dividing embryonic and imaginal disc cells. It recognizes the product of the peanut gene, which encodes a protein similar to the bud neck filament proteins of S. cerevisiae (CDC3, CDC10, CDC11 and CDC12).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Peanut is a septin
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C9H4 anti-peanut was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 4C9H4 anti-peanut)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins.
Rubin GM
Cell 77.3 (1994 May 6): 371-9.