3H5

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SKU: 3H5

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 3H5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_2314644 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Chicken optic nerve (E10)
Clone:
Immunogen Sequence:
Myeloma Strain: X63Ag8.653
Epitope Mapped: Yes
Antigen Name: HNK-1 carbohydrate epitope
Epitope Location or Sequence: SO4-3GlcAb1-3Galb1-4GlcNAc
Alternate Antigen Name:
Deposit Date: 9/14/2009
Antigen Molecular Weight:
Depositor: Halfter, W.M.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: chicken
Depositor Notes: The HNK-1 epitope is a N-linked carbohydrate marker of neural crest cells and of a subset of lymphocytes. This marker is also known as CD57.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Lamprey, Shark
Additional Information: 3H5 is a mouse hybridoma that was generated against chick optic nerve, and is not a subclone of the VC1.1 hybridoma which is a mouse IgM and also recognizes the HNK-1 epitope.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H5 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum.
    De Bellard ME
    The Journal of comparative neurology 521.14 (2013 Oct 1): 3303-20.

    IF References

    Neural crest stem cells and their potential therapeutic applications.
    Cheung M
    Developmental biology 419.2 (2016 Nov 15): 199-216.

    Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum.
    De Bellard ME
    The Journal of comparative neurology 521.14 (2013 Oct 1): 3303-20.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    A fate-map for cranial sensory ganglia in the sea lamprey.
    Baker CV
    Developmental biology 385.2 (2014 Jan 15): 405-16.

    IHC References

    Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube.
    Bronner ME
    Molecular biology of the cell 25.3 (2014 Feb): 347-55.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Elk3 is essential for the progression from progenitor to definitive neural crest cell.
    Bronner ME
    Developmental biology 374.2 (2013 Feb 15): 255-63.

    Cxcr4 and Sdf-1 are critically involved in the formation of facial and non-somitic neck muscles.
    Morosan-Puopolo G
    Scientific reports 10.1 (2020 Mar 19): 5049.

    Clonal analysis and dynamic imaging identify multipotency of individual Gallus gallus caudal hindbrain neural crest cells toward cardiac and enteric fates.
    Bronner ME
    Nature communications 12.1 (2021 Mar 25): 1894.

    The development of the trunk neural crest in the turtle Trachemys scripta.
    de Bellard ME
    Developmental dynamics : an official publication of the American Association of Anatomists 249.1 (2020 Jan): 125-140.

    All References

    Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube.
    Bronner ME
    Molecular biology of the cell 25.3 (2014 Feb): 347-55.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Elk3 is essential for the progression from progenitor to definitive neural crest cell.
    Bronner ME
    Developmental biology 374.2 (2013 Feb 15): 255-63.

    Cxcr4 and Sdf-1 are critically involved in the formation of facial and non-somitic neck muscles.
    Morosan-Puopolo G
    Scientific reports 10.1 (2020 Mar 19): 5049.

    Clonal analysis and dynamic imaging identify multipotency of individual Gallus gallus caudal hindbrain neural crest cells toward cardiac and enteric fates.
    Bronner ME
    Nature communications 12.1 (2021 Mar 25): 1894.

    The development of the trunk neural crest in the turtle Trachemys scripta.
    de Bellard ME
    Developmental dynamics : an official publication of the American Association of Anatomists 249.1 (2020 Jan): 125-140.

    Neural crest stem cells and their potential therapeutic applications.
    Cheung M
    Developmental biology 419.2 (2016 Nov 15): 199-216.

    Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum.
    De Bellard ME
    The Journal of comparative neurology 521.14 (2013 Oct 1): 3303-20.

    A fate-map for cranial sensory ganglia in the sea lamprey.
    Baker CV
    Developmental biology 385.2 (2014 Jan 15): 405-16.

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