22d4
Catalog Fields
Clone ID/Product Name: 22d4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: M6PR
Ab Isotype: MIgG2b, kappa light chain
Gene Name:
Entrez Gene ID: 281291
Clonality: Monoclonal
Immunogen: Mannose 6-phosphate receptors mix (MPR) purified from bovine calf liver
Clone:
Immunogen Sequence:
Myeloma Strain: P3-X63-Ag8.653.3
Epitope Mapped: Yes
Antigen Name: mannose 6-phosphate receptor, cation-dependent (M6PR)
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 10/21/2004
Antigen Molecular Weight: Predicted: 28 kDa; Apparent: 46 kDa
Depositor: Messner, D.
Antigen Sequence:
Depositor Institution: Bastyr University
Antigen Species: bovine
Depositor Notes: Use non-reducing conditions for western blotting. 22d4 doesn't recognize the murine M6PR.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Human
Additional Information: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22d4 was deposited to the DSHB by Messner, D. (DSHB Hybridoma Product 22d4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
Messner DJ
Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.