1h5
Catalog Fields
Clone ID/Product Name: 1h5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528323
Clonality: Monoclonal
Immunogen: insoluble residues of Xenopus A6 (kidney epithelial) cells
Clone:
Immunogen Sequence:
Myeloma Strain: P3
Epitope Mapped: No
Antigen Name: cytokeratin type II (Xenopus)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/1/1988
Antigen Molecular Weight: 59 kDa
Depositor: Klymkowsky, M.
Antigen Sequence:
Depositor Institution: University of Colorado / MCD Biology
Antigen Species: Xenopus
Depositor Notes: The antibody induces the disruption of cytokeratin organization when injected into A6 cells. no dramatic effect when injected into embryos. Immunohistochemistry stains cortical cytokeratin system of early Xenoopus embryo. The specificity of the antibody is fro type II cytokeratins of Xenopus early embryo.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Xenopus
Additional Information: The immunization route is multiple/intraperitoneal using RIBI adjuvant. RRID:AB_528323
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1h5 was deposited to the DSHB by Klymkowsky, M. (DSHB Hybridoma Product 1h5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
2 References
Initial Publication
Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos.
Polson AG
Development (Cambridge, England) 100.3 (1987 Jul): 543-57.