14h7
Catalog Fields
Product Name/ID: 14h7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: vim1 & vim4
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528507
Uniprot ID: P24789 P24790
Entrez Gene ID: 386601 386600
Clonality: Monoclonal
Immunogen: Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3
Epitope Mapped: No
Antigen Name: vimentin (Xenopus)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/1/1988
Antigen Molecular Weight: Predicted: 52.8 (vim1) and 53.5 (vim4) kDa; Apparent: 55 (vim1) and 57 (vim4) kDa
Depositor: Klymkowsky, M.
Antigen Sequence:
Depositor Institution: University of Colorado / MCD Biology
Antigen Species: Xenopus
Depositor Notes: This antibody appears to be Xenopus-specific and doesn't cross react with mouse. It recognizes the 55 and 57 kDa vimentin proteins encoded by the genes vim1 and vim4, respectively. 14h7 disrupts vimentin organization when injected into Xenopus A6 cells. 14H7 undergoes frequent class switching from IgG1 to IgG2a.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Xenopus
Additional Information: While this antibody has been reported to be Xenopus-specific, Arciniegas et al. [PMID: 10603448] and Gomes et al. [PMID: 22075471] have reported using it succesfully on chicken embryos and mouse cultured ectoplacental cones, respectively.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14h7 was deposited to the DSHB by Klymkowsky, M. (DSHB Hybridoma Product 14h7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos.
Polson AG
Development (Cambridge, England) 100.3 (1987 Jul): 543-57.