N3.36
Catalog Fields
Product Name/ID: N3.36
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYH8
Ab Isotype: MIgM
Gene Symbol:
Entrez Gene ID: 4626
Clonality: Monoclonal
Immunogen: partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/2
Epitope Mapped: Yes
Antigen Name: Myosin heavy chain Type IIA fast and human neonatal
Epitope Location or Sequence: Heavy meromyosin subfragment
Alternate Antigen Name:
Deposit Date: 8/25/1993
Antigen Molecular Weight: 220kDa
Depositor: Blau, H.M.
Antigen Sequence:
Depositor Institution: Stanford University, Baxter Lab for Stem Cell Biology
Antigen Species: Human
Depositor Notes: Fusion: 1983. Recognizes neonatal and adult fast fibers.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Human, Rat, Rodent
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N3.36 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product N3.36)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Developmental progression of myosin gene expression in cultured muscle cells.
Blau HM
Cell 46.7 (1986 Sep 26): 1075-81.