F5D
Catalog Fields
Product Name/ID: F5D
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Myog
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2146602
Uniprot ID: P20428
Entrez Gene ID: 29148
Clonality: Monoclonal
Immunogen: GST-fusion protein
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Myogenin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/10/1996
Antigen Molecular Weight: Predicted: 24kDa; apparent: 34 kDa
Depositor: Wright, W.E.
Antigen Sequence:
Depositor Institution: University of Texas Southwestern Medical Center
Antigen Species: Rat
Depositor Notes: This antibody immunoprecipitates deletion mutants containing a.a. 138-158, region immediately carboxy-terminal to the bHLH domain.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Feline, Human, Mammal, Mouse, Porcine, Rat
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F5D was deposited to the DSHB by Wright, W.E. (DSHB Hybridoma Product F5D)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
35 References
Initial Publication
Cyclic amplification and selection of targets (CASTing) for the myogenin consensus binding site.
Funk W
Molecular and cellular biology 11.8 (1991 Aug): 4104-10.