10F5
Catalog Fields
Product Name/ID: 10F5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYH4
Ab Isotype: MIgM, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_1157896
Uniprot ID: Q28641
Entrez Gene ID: 100125991
Clonality: Monoclonal
Immunogen: rabbit retractor bulbi muscle
Clone:
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: Myosin heavy chain, Type IIB
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/26/2007
Antigen Molecular Weight: Predicted: 223kDa
Depositor: Lucas, C.
Antigen Sequence: myosin
Depositor Institution: University of Sydney
Antigen Species: Rabbit
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Antechinus, Bandicoot, Bettongia, Cheetah, Guinea Pig, Human, Kangaroo, Mouse, Porcine, Pseudocheirus, Rabbit, Rat, Trichosurus, Wallaby
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10F5 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 10F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
27 References
Initial Publication
Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
Hoh JF
Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.