poly(GP) TALS 828.179
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Catalog Fields
Clone ID/Product Name: poly(GP) TALS 828.179
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: C9ORF72
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2753317
Uniprot ID: Q96LT7
Entrez Gene ID: 203228
Clonality: Monoclonal
Immunogen: H2N-C(DPEG4) GPGPGPGPGPGPGPGP
Clone:
Immunogen Sequence: GPGPGPGPGPGPGPGP
Myeloma Strain:
Epitope Mapped: No
Antigen Name: GP dipeptide repeat proteins, poly(GP)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/3/2018
Antigen Molecular Weight: The molecular weight will depend on the number of GP dipeptide repeats making up the protein.
Depositor: Target ALS Foundation
Antigen Sequence:
Depositor Institution: Target ALS Foundation
Antigen Species: Human
Depositor Notes: This antibody recognizes poly(GP) dipeptide repeat proteins produced from an expanded hexanucleotide (GGGGCC) repeat in the first intron of C9ORF72, the most common known genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The expanded GGGGCC repeat undergoes an atypical form of translation - repeat-associated non-ATG (RAN) translation - forming various dipeptide repeat proteins, one of which is poly(GP). The hybridoma was commercially generated with funds from the Target ALS Foundation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
poly(GP) TALS 828.179 was deposited to the DSHB by Target ALS Foundation (DSHB Hybridoma Product poly(GP) TALS 828.179)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
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