osa
Catalog Fields
Clone ID/Product Name: osa
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: OSA
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 42130
Clonality: Monoclonal
Immunogen: GST fusion protein with OSA ARID domain
Clone:
Immunogen Sequence: a.a. 965-1049
Myeloma Strain: P3XAg8.653
Epitope Mapped: No
Antigen Name: Osa,trithorax group protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/1/2002
Antigen Molecular Weight: 284 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: This monoclonal antibody was originally named 15A8. Osa protein is also known as eyelid protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: FBgn0261885. RRID: AB_528420
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
osa was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product osa)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
eyelid antagonizes wingless signaling during Drosophila development and has homology to the Bright family of DNA-binding proteins.
Heberlein U
Genes & development 11.15 (1997 Aug 1): 1949-62.