anti-dFmr1, 5A11
Catalog Fields
Clone ID/Product Name: anti-dFmr1, 5A11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Fmr1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 37528
Clonality: Monoclonal
Immunogen: Recombinant His-tagged dFmr1 protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: No
Antigen Name: dFmr1 protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/5/2003
Antigen Molecular Weight: Multiple isforms; Predicted: between 60 and 76 kDa
Depositor: Siomi, H.
Antigen Sequence:
Depositor Institution: Keio University School of Medicine
Antigen Species: Drosophila
Depositor Notes: MW: 78 kDa (+82 kDa minor band)
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-dFmr1, 5A11 was deposited to the DSHB by Siomi, H. (DSHB Hybridoma Product anti-dFmr1, 5A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways.
Siomi MC
Genes & development 18.14 (2004 Jul 15): 1655-66.