anti-HA rRb-IgG
Catalog Fields
Product Name/ID: anti-HA rRb-IgG
Available to For-Profits: Yes
Alternate Antibody Name: anti-HA
Gene Name: HA
Ab Isotype: Rabbit IgG
Gene Symbol:
Uniprot ID: P03435
Clonality: Monoclonal
Immunogen: Synthetic Peptide conjugated to KLH
Clone:
Immunogen Sequence: a.a. 75-110
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Hemagglutinin
Epitope Location or Sequence: YPYDVPDYA
Alternate Antigen Name:
Deposit Date: 8/12/2022
Antigen Molecular Weight: 1.1 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Influenza A virus strain A/Victoria/3/1975 H3N2
Depositor Notes: This antibody contains the 12CA5 paratope.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: All
Additional Information:
Additional Characterization: https://www.rcsb.org/structure/5XCS
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-HA rRb-IgG was deposited to the DSHB by DSHB (DSHB Hybridoma Product anti-HA rRb-IgG)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
The structure of an antigenic determinant in a protein.
Lerner RA
Cell 37.3 (1984 Jul): 767-78.