anti-Antp 4C3

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SKU: anti-Antp 4C3

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DSHB Data Sheet
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Clone ID/Product Name: anti-Antp 4C3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Antp
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528082 
Uniprot ID: P02833 
RRID:  
Entrez Gene ID: 40835 
Clonality: Monoclonal
Immunogen: lacZ-Antp fusion protein
Clone:
Immunogen Sequence:
Myeloma Strain: NSO
Epitope Mapped: No
Antigen Name: Antennapedia protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2002
Antigen Molecular Weight: Predicted: 43kDa (multiple isoforms from 33 to 43kDa)
Depositor: Brower, D.
Antigen Sequence:
Depositor Institution: University of Arizona
Antigen Species: Drosophila
Depositor Notes: Species Specificity: shows broad species binding.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Beetle, Broad species, Drosophila, Grasshopper
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Antp 4C3 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Antp 4C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

35 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Gel Supershift References
  • All References
    • Initial Publication

    [Demonstration of tumor inhibiting properties of a strongly immunostimulating low-molecular weight substance. Comparative studies with ifosfamide on the immuno-labile DS carcinosarcoma. Stimulation of the autoimmune activity for approx. 20 days by BA 1, a N-(2-cyanoethylene)-urea. Novel prophylactic possibilities].
    Reitnauer PG
    Arzneimittel-Forschung 25.9 (1975 Sep): 1369-79.

    IF References

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.
    Tsuda L
    Genes to cells : devoted to molecular & cellular mechanisms 18.11 (2013 Nov): 909-20.

    The homeobox gene cut interacts genetically with the homeotic genes proboscipedia and Antennapedia.
    Blochlinger K
    Genetics 149.1 (1998 May): 131-42.

    Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis.
    Schulz RA
    Development (Cambridge, England) 137.21 (2010 Nov): 3561-8.

    bantam miRNA is important for Drosophila blood cell homeostasis and a regulator of proliferation in the hematopoietic progenitor niche.
    Schulz RA
    Biochemical and biophysical research communications 453.3 (2014 Oct 24): 467-72.

    Asrij maintains the stem cell niche and controls differentiation during Drosophila lymph gland hematopoiesis.
    Inamdar MS
    PloS one 6.11 (2011): e27667.

    A unique Extradenticle recruitment mode in the Drosophila Hox protein Ultrabithorax.
    Graba Y
    Proceedings of the National Academy of Sciences of the United States of America 104.43 (2007 Oct 23): 16946-51.

    Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity.
    Thor S
    Development (Cambridge, England) 131.24 (2004 Dec): 6093-105.

    Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland.
    Schulz RA
    PloS one 7.7 (2012): e41604.

    Additional sex combs affects antennal development by means of spatially restricted repression of Antp and wg.
    Salzberg A
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2118-30.

    A single origin for nymphalid butterfly eyespots followed by widespread loss of associated gene expression.
    Monteiro A
    PLoS genetics 8.8 (2012): e1002893.

    Cross-regulatory protein-protein interactions between Hox and Pax transcription factors.
    Gehring WJ
    Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008 Sep 9): 13439-44.

    Over-expression of Ultrabithorax alters embryonic body plan and wing patterns in the butterfly Bicyclus anynana.
    Monteiro A
    Developmental biology 394.2 (2014 Oct 15): 357-66.

    Insights into Hox protein function from a large scale combinatorial analysis of protein domains.
    Graba Y
    PLoS genetics 7.10 (2011 Oct): e1002302.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.
    Alonso CR
    Development (Cambridge, England) 141.10 (2014 May): 2046-56.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
    Tanentzapf G
    Current biology : CB 30.17 (2020 Sep 7): 3316-3329.e5.

    Hox dosage contributes to flight appendage morphology in Drosophila.
    Merabet S
    Nature communications 12.1 (2021 May 17): 2892.

    WB References

    The YPWM motif links Antennapedia to the basal transcriptional machinery.
    Gehring WJ
    Development (Cambridge, England) 135.9 (2008 May): 1669-79.

    IHC References

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Drosophila midgut morphogenesis requires the function of the segmentation gene odd-paired.
    Sakonju S
    Developmental biology 169.2 (1995 Jun): 580-95.

    The Tribolium homeotic gene Abdominal is homologous to abdominal-A of the Drosophila bithorax complex.
    Denell RE
    Development (Cambridge, England) 117.1 (1993 Jan): 233-43.

    Sequence and expression of grasshopper antennapedia: comparison to Drosophila.
    Ball EE
    Developmental biology 172.2 (1995 Dec): 452-65.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Expression and function of the homoeotic genes Antennapedia and Sex combs reduced in the embryonic midgut of Drosophila.
    Scott MP
    Development (Cambridge, England) 109.2 (1990 Jun): 289-303.

    Trithorax regulates multiple homeotic genes in the bithorax and Antennapedia complexes and exerts different tissue-specific, parasegment-specific and promoter-specific effects on each.
    Harte PJ
    Development (Cambridge, England) 117.1 (1993 Jan): 119-34.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    Ectopic expression and function of the Antp and Scr homeotic genes: the N terminus of the homeodomain is critical to functional specificity.
    Scott MP
    Development (Cambridge, England) 118.2 (1993 Jun): 339-52.

    A Synthetic Peptide, CK2.3, Inhibits RANKL-Induced Osteoclastogenesis through BMPRIa and ERK Signaling Pathway.
    Nohe A
    Journal of developmental biology 8.3 (2020 Jul 9): .

    Gel Supershift References

    Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori.
    Takiya S
    Developmental biology 386.1 (2014 Feb 1): 64-71.

    All References

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Drosophila midgut morphogenesis requires the function of the segmentation gene odd-paired.
    Sakonju S
    Developmental biology 169.2 (1995 Jun): 580-95.

    The Tribolium homeotic gene Abdominal is homologous to abdominal-A of the Drosophila bithorax complex.
    Denell RE
    Development (Cambridge, England) 117.1 (1993 Jan): 233-43.

    Sequence and expression of grasshopper antennapedia: comparison to Drosophila.
    Ball EE
    Developmental biology 172.2 (1995 Dec): 452-65.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Expression and function of the homoeotic genes Antennapedia and Sex combs reduced in the embryonic midgut of Drosophila.
    Scott MP
    Development (Cambridge, England) 109.2 (1990 Jun): 289-303.

    Trithorax regulates multiple homeotic genes in the bithorax and Antennapedia complexes and exerts different tissue-specific, parasegment-specific and promoter-specific effects on each.
    Harte PJ
    Development (Cambridge, England) 117.1 (1993 Jan): 119-34.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    Ectopic expression and function of the Antp and Scr homeotic genes: the N terminus of the homeodomain is critical to functional specificity.
    Scott MP
    Development (Cambridge, England) 118.2 (1993 Jun): 339-52.

    A Synthetic Peptide, CK2.3, Inhibits RANKL-Induced Osteoclastogenesis through BMPRIa and ERK Signaling Pathway.
    Nohe A
    Journal of developmental biology 8.3 (2020 Jul 9): .

    Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.
    Tsuda L
    Genes to cells : devoted to molecular & cellular mechanisms 18.11 (2013 Nov): 909-20.

    The homeobox gene cut interacts genetically with the homeotic genes proboscipedia and Antennapedia.
    Blochlinger K
    Genetics 149.1 (1998 May): 131-42.

    Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis.
    Schulz RA
    Development (Cambridge, England) 137.21 (2010 Nov): 3561-8.

    bantam miRNA is important for Drosophila blood cell homeostasis and a regulator of proliferation in the hematopoietic progenitor niche.
    Schulz RA
    Biochemical and biophysical research communications 453.3 (2014 Oct 24): 467-72.

    Asrij maintains the stem cell niche and controls differentiation during Drosophila lymph gland hematopoiesis.
    Inamdar MS
    PloS one 6.11 (2011): e27667.

    A unique Extradenticle recruitment mode in the Drosophila Hox protein Ultrabithorax.
    Graba Y
    Proceedings of the National Academy of Sciences of the United States of America 104.43 (2007 Oct 23): 16946-51.

    Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity.
    Thor S
    Development (Cambridge, England) 131.24 (2004 Dec): 6093-105.

    Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland.
    Schulz RA
    PloS one 7.7 (2012): e41604.

    Additional sex combs affects antennal development by means of spatially restricted repression of Antp and wg.
    Salzberg A
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2118-30.

    A single origin for nymphalid butterfly eyespots followed by widespread loss of associated gene expression.
    Monteiro A
    PLoS genetics 8.8 (2012): e1002893.

    Cross-regulatory protein-protein interactions between Hox and Pax transcription factors.
    Gehring WJ
    Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008 Sep 9): 13439-44.

    Over-expression of Ultrabithorax alters embryonic body plan and wing patterns in the butterfly Bicyclus anynana.
    Monteiro A
    Developmental biology 394.2 (2014 Oct 15): 357-66.

    Insights into Hox protein function from a large scale combinatorial analysis of protein domains.
    Graba Y
    PLoS genetics 7.10 (2011 Oct): e1002302.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.
    Alonso CR
    Development (Cambridge, England) 141.10 (2014 May): 2046-56.

    Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
    Tanentzapf G
    Current biology : CB 30.17 (2020 Sep 7): 3316-3329.e5.

    Hox dosage contributes to flight appendage morphology in Drosophila.
    Merabet S
    Nature communications 12.1 (2021 May 17): 2892.

    The YPWM motif links Antennapedia to the basal transcriptional machinery.
    Gehring WJ
    Development (Cambridge, England) 135.9 (2008 May): 1669-79.

    [Demonstration of tumor inhibiting properties of a strongly immunostimulating low-molecular weight substance. Comparative studies with ifosfamide on the immuno-labile DS carcinosarcoma. Stimulation of the autoimmune activity for approx. 20 days by BA 1, a N-(2-cyanoethylene)-urea. Novel prophylactic possibilities].
    Reitnauer PG
    Arzneimittel-Forschung 25.9 (1975 Sep): 1369-79.

    Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori.
    Takiya S
    Developmental biology 386.1 (2014 Feb 1): 64-71.

    Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
    Gould AP
    PLoS biology 6.3 (2008 Mar 11): e58.

    Drosophila retinal pigment cell death is regulated in a position-dependent manner by a cell memory gene.
    Théodore L
    The International journal of developmental biology 52.1 (2008): 21-31.

    Polycomb genes interact with the tumor suppressor genes hippo and warts in the maintenance of Drosophila sensory neuron dendrites.
    Jan YN
    Genes & development 21.8 (2007 Apr 15): 956-72.

    Evolving role of Antennapedia protein in arthropod limb patterning.
    Hayashi S
    Development (Cambridge, England) 129.15 (2002 Aug): 3555-61.

    Homeotic genes regulate the spatial expression of putative growth factors in the visceral mesoderm of Drosophila embryos.
    Scott MP
    Development (Cambridge, England) 110.4 (1990 Dec): 1031-40.

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