TS1/18.1.2.11.4
Catalog Fields
Clone ID/Product Name: TS1/18.1.2.11.4
Available to For-Profits: Yes
Alternate Antibody Name: TS1/18
Gene Symbol: ITGB2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2128634
Uniprot ID: P05107
Entrez Gene ID: 3689
Clonality: Monoclonal
Immunogen: Human HLA-DRCTL line cytolytic T-lymphocytes.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Antigen Name: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/4/1989
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Center for Blood Research
Antigen Species: Human
Depositor Notes: Does not cross react with mouse. Binds to all leukocytes. Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: CD18 is a general marker of leukocytes.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1/18.1.2.11.4 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product TS1/18.1.2.11.4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
Springer TA
Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.