P4A4
Catalog Fields
Clone ID/Product Name: P4A4
Available to For-Profits: Yes
Alternate Antibody Name: CD105
Gene Symbol: ENG
Ab Isotype: MIgG2b, kappa light chain
Gene Name:
Entrez Gene ID: 2022
Clonality: Monoclonal
Immunogen: Human unbilical vein endothelial cells
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: NS-I/FOX-NY
Epitope Mapped: Yes
Antigen Name: Endoglin/CD105/TGF-receptor III complex
Epitope Location or Sequence: a.a. 277-331
Alternate Antigen Name:
Deposit Date: 2/12/1997
Antigen Molecular Weight: 90 kDa (monomer) and 180 kDa (dimer)
Depositor: Wayner, E.A. / Vercellotti, G.
Antigen Sequence:
Depositor Institution: University of Minnesota
Antigen Species: Human
Depositor Notes: P4A4 may also be used to activate T cell binding to endothelium.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Monkey, Mouse, Porcine, Rat
Additional Information: RRID: AB_528222
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4A4 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P4A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Mapping epitopes to distinct regions of the extracellular domain of endoglin using bacterially expressed recombinant fragments.
Letarte M
Tissue antigens 50.3 (1997 Sep): 265-76.