P2C62C4
Catalog Fields
Clone ID/Product Name: P2C62C4
Available to For-Profits: Yes
Alternate Antibody Name: alpha6C6
Gene Symbol: ITGA6
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 396226
Clonality: Monoclonal
Immunogen: Denatured integrin alpha subunits purified from adult chicken brain tissue.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: integrin alpha-6, avian
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/20/1993
Antigen Molecular Weight: Predicted: 119 kDa; Apparent: 130 kDa
Depositor: Horwitz, A.F.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Chicken
Depositor Notes: This antibody has been generated against denatured protein; it immunoblots well and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but doesn't precipitate the native receptor. Stains chicken embryo cryosections.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information: P2C62C4 recognizes both uncleaved (proform) and cleaved forms of alpha-6 integrin.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2C62C4 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product P2C62C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Developmentally regulated expression of alpha 6 integrin in avian embryos.
Horwitz AF
Development (Cambridge, England) 115.1 (1992 May): 197-211.