OX-CH1
Catalog Fields
Clone ID/Product Name: OX-CH1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_2617415
Clonality: Monoclonal
Immunogen: Immunogen prepared at Plant Sciences, University of Oxford, UK from PBS, cell free surface washings (5 ml) of 14-day-old cultures of Cladosporium herbarum race 4
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: glycoprotein, Cladosporium herbarum race 4
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/9/2010
Antigen Molecular Weight:
Depositor: Dewey, F.M.
Antigen Sequence:
Depositor Institution: University of Oxford
Antigen Species: Cladosporium
Depositor Notes: glycoprotein, antibody binds to carbohydrate epitope which is thermostable, resistant to protease but destroyed by deglycosylation. Cell binding: binds to water soluble product produced by mycelium of Cladosporium species grown in vitro and in planta. near-genus-specific, recognizes all species Cladosporium (fungi) in vitro and in planta, but does not recognize other fungi including yeasts or extracts from uninfected plants. Functional effects: has been used to determine, by ELISA, the biomass of C. herbarum in infected tomato leaves.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Cladosporium
Additional Information:
Recommended Applications: ELISA
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
OX-CH1 was deposited to the DSHB by Dewey, F.M. (DSHB Hybridoma Product OX-CH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Use of monoclonal antibodies to determine biomass of Cladosporium fulvum in infected tomato leaves.
Dewey FM
Molecular plant-microbe interactions : MPMI 11.7 (1998 Jul): 710-6.