N76/8
Catalog Fields
Clone ID/Product Name: N76/8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Atxn1
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2877293
Uniprot ID: P54254
Entrez Gene ID: 20238
Clonality: Monoclonal
Immunogen: Synthetic peptide
Clone:
Immunogen Sequence: a.a. 164-197
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Ataxin-1, 11NQ
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/8/2022
Antigen Molecular Weight: 85 kD
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Mouse
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information: Validated by KO.
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N76_8.pdf
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N76/8 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N76/8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
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