N294A/10
Catalog Fields
Clone ID/Product Name: N294A/10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Bcan
Ab Isotype: MIgG2a
Gene Name:
Uniprot ID: P55068
Entrez Gene ID: 25393
Clonality: Monoclonal
Immunogen: Fusion protein
Clone:
Immunogen Sequence: a.a. 219-655
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Brevican core protein
Epitope Location or Sequence: core protein
Alternate Antigen Name:
Deposit Date: 1/4/2023
Antigen Molecular Weight: 96.0 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Rat
Depositor Notes: May stabilize interactions between hyaluronan and brain proteoglycans.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N294A_10.pdf
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N294A/10 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N294A/10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
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